Mining of camel rumen metagenome to identify novel alkali-thermostable xylanase capable of enhancing the recalcitrant lignocellulosic biomass conversion

Shohreh Ariaeenejad; Morteza Maleki; Elnaz Hosseini; Kaveh Kavousi; Ali A. Moosavi-Movahedi; Ghasem Hosseini Salekdeh

doi:10.1016/j.biortech.2019.02.059

Mining of camel rumen metagenome to identify novel alkali-thermostable xylanase capable of enhancing the recalcitrant lignocellulosic biomass conversion

Shohreh Ariaeenejad, Morteza Maleki, Elnaz Hosseini, Kaveh Kavousi, Ali A. Moosavi-Movahedi, Ghasem Hosseini Salekdeh^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

The aim of this study was to isolate and characterize novel alkali-thermostable xylanase genes from the mixed genome DNA of camel rumen metagenome. In this study, a five-stage computational screening procedure was utilized to find the primary candidate enzyme with superior properties from the camel rumen metagenome. This enzyme was subjected to cloning, purification, and structural and functional characterization. It showed high thermal stability, high activity in a broad range of pH (6–11) and temperature (30–90 °C) and effectivity in recalcitrant lignocellulosic biomass degradation. Our results demonstrated the power of in silico analysis to discover novel alkali-thermostable xylanases, effective for the bioconversion of lignocellulosic biomass.

Original language	English
Pages (from-to)	343-350
Number of pages	8
Journal	Bioresource Technology
Volume	281
DOIs	https://doi.org/10.1016/j.biortech.2019.02.059
Publication status	Published - Jun 2019
Externally published	Yes

Keywords

Alkaline thermostable xylanase
Metagenome
Camel rumen
Recalcitrant compounds

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10.1016/j.biortech.2019.02.059

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title = "Mining of camel rumen metagenome to identify novel alkali-thermostable xylanase capable of enhancing the recalcitrant lignocellulosic biomass conversion",

abstract = "The aim of this study was to isolate and characterize novel alkali-thermostable xylanase genes from the mixed genome DNA of camel rumen metagenome. In this study, a five-stage computational screening procedure was utilized to find the primary candidate enzyme with superior properties from the camel rumen metagenome. This enzyme was subjected to cloning, purification, and structural and functional characterization. It showed high thermal stability, high activity in a broad range of pH (6–11) and temperature (30–90 °C) and effectivity in recalcitrant lignocellulosic biomass degradation. Our results demonstrated the power of in silico analysis to discover novel alkali-thermostable xylanases, effective for the bioconversion of lignocellulosic biomass.",

keywords = "Alkaline thermostable xylanase, Metagenome, Camel rumen, Recalcitrant compounds",

author = "Shohreh Ariaeenejad and Morteza Maleki and Elnaz Hosseini and Kaveh Kavousi and Moosavi-Movahedi, {Ali A.} and Salekdeh, {Ghasem Hosseini}",

year = "2019",

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doi = "10.1016/j.biortech.2019.02.059",

language = "English",

volume = "281",

pages = "343--350",

journal = "Bioresource Technology",

issn = "0960-8524",

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AU - Ariaeenejad, Shohreh

AU - Maleki, Morteza

AU - Hosseini, Elnaz

AU - Kavousi, Kaveh

AU - Moosavi-Movahedi, Ali A.

AU - Salekdeh, Ghasem Hosseini

PY - 2019/6

Y1 - 2019/6

N2 - The aim of this study was to isolate and characterize novel alkali-thermostable xylanase genes from the mixed genome DNA of camel rumen metagenome. In this study, a five-stage computational screening procedure was utilized to find the primary candidate enzyme with superior properties from the camel rumen metagenome. This enzyme was subjected to cloning, purification, and structural and functional characterization. It showed high thermal stability, high activity in a broad range of pH (6–11) and temperature (30–90 °C) and effectivity in recalcitrant lignocellulosic biomass degradation. Our results demonstrated the power of in silico analysis to discover novel alkali-thermostable xylanases, effective for the bioconversion of lignocellulosic biomass.

AB - The aim of this study was to isolate and characterize novel alkali-thermostable xylanase genes from the mixed genome DNA of camel rumen metagenome. In this study, a five-stage computational screening procedure was utilized to find the primary candidate enzyme with superior properties from the camel rumen metagenome. This enzyme was subjected to cloning, purification, and structural and functional characterization. It showed high thermal stability, high activity in a broad range of pH (6–11) and temperature (30–90 °C) and effectivity in recalcitrant lignocellulosic biomass degradation. Our results demonstrated the power of in silico analysis to discover novel alkali-thermostable xylanases, effective for the bioconversion of lignocellulosic biomass.

KW - Alkaline thermostable xylanase

KW - Metagenome

KW - Camel rumen

KW - Recalcitrant compounds

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SN - 0960-8524

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JO - Bioresource Technology

JF - Bioresource Technology

ER -

Mining of camel rumen metagenome to identify novel alkali-thermostable xylanase capable of enhancing the recalcitrant lignocellulosic biomass conversion

Abstract

Keywords

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