Mink serum amyloid a protein – expression and primary structure of amyloidogenic and non-amyloidogenic isotypes

G. Marhaug, G. Husby, K. Nordstoga, S.B. Dowton

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contribution

Abstract

When Northern blot analysis of LPS stimulated mink liver RNA was performed using a SAA specific probe, several signals were found. Nucleotide sequence analysis of two clones from a cDNA library was performed. Deduced amino acid sequence of the longer one, SAA1 (776 bp), indicates that it represents a SAA isotype found only in serum, while the shorter one, SAA2 (552 bp), corresponds to an isotype found both in serum and degraded to protein AA in secondary amyloid. These results show that the situation in mink is the same as in mice, where only one out of at least two SAA isotypes has been shown to be amyloidogenic. Northern blot analysis also showed that almost all SAA mRNA was found in the liver, while only minor amounts of SAA1 and SAA2 mRNA was found in the brain and the lungs. No signals could be detected in amyloid prone organs like intestine and spleen. In the liver a SAA specific signal was found corresponding to 2.2 kb, and after in vitro translation a protein SAA band corresponding to 28,000 Daltons was detected, indicating that a SAA isotype with high molecular weight exists in this animal.
Original languageEnglish
Title of host publicationAmyloid and amyloidosis 1990
Subtitle of host publicationVIth international symposium on amyloidosis August 5–8, 1990, Oslo, Norway
EditorsJacob B. Natvig, Øystein Førre, Gunnar Husby, Anne Husebekk, Bjørn Skogen, Knut Sletten, Per Westermark
Place of PublicationNetherlands
PublisherSpringer, Springer Nature
Pages36-39
Number of pages4
ISBN (Electronic)9789401132848
ISBN (Print)9789401054508
DOIs
Publication statusPublished - 1991
Externally publishedYes
EventInternational Symposium on Amyloidosis (6th : 1990) - Oslo, Norway
Duration: 5 Aug 19908 Aug 1990

Conference

ConferenceInternational Symposium on Amyloidosis (6th : 1990)
CityOslo, Norway
Period5/08/908/08/90

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  • Cite this

    Marhaug, G., Husby, G., Nordstoga, K., & Dowton, S. B. (1991). Mink serum amyloid a protein – expression and primary structure of amyloidogenic and non-amyloidogenic isotypes. In J. B. Natvig, Ø. Førre, G. Husby, A. Husebekk, B. Skogen, K. Sletten, & P. Westermark (Eds.), Amyloid and amyloidosis 1990: VIth international symposium on amyloidosis August 5–8, 1990, Oslo, Norway (pp. 36-39). Netherlands: Springer, Springer Nature. https://doi.org/10.1007/978-94-011-3284-8_9