miR-16 and miR-103 impact 5-HT 4 receptor signalling and correlate with symptom profile in irritable bowel syndrome

Carolin Wohlfarth, Stefanie Schmitteckert, Janina D. Härtle, Lesley A. Houghton, Harsh Dweep, Marina Fortea, Ghazaleh Assadi, Alexander Braun, Tanja Mederer, Sarina Pöhner, Philip P. Becker, Christine Fischer, Martin Granzow, Hubert Mönnikes, Emeran A. Mayer, Gregory Sayuk, Guy Boeckxstaens, Mira M. Wouters, Magnus Simrén, Greger LindbergBodil Ohlsson, Peter Thelin Schmidt, Aldona Dlugosz, Lars Agreus, Anna Andreasson, Mauro D'Amato, Barbara Burwinkel, Justo Lorenzo Bermejo, Ralph Röth, Felix Lasitschka, Maria Vicario, Marco Metzger, Javier Santos, Gudrun A. Rappold, Cristina Martinez, Beate Niesler*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)
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Abstract

Irritable bowel syndrome (IBS) is a gut-brain disorder involving alterations in intestinal sensitivity and motility. Serotonin 5-HT 4 receptors are promising candidates in IBS pathophysiology since they regulate gut motor function and stool consistency, and targeted 5-HT 4 R selective drug intervention has been proven beneficial in subgroups of patients. We identified a single nucleotide polymorphism (SNP) (rs201253747) c.∗61 T > C within the 5-HT 4 receptor gene HTR4 to be predominantly present in diarrhoea-IBS patients (IBS-D). It affects a binding site for the miR-16 family and miR-103/miR-107 within the isoforms HTR4b/i and putatively impairs HTR4 expression. Subsequent miRNA-profiling revealed downregulation of miR-16 and miR-103 in the jejunum of IBS-D patients correlating with symptoms. In vitro assays confirmed expression regulation via three 3′UTR binding sites. The novel isoform HTR4b-2 lacking two of the three miRNA binding sites escapes miR-16/103/107 regulation in SNP carriers. We provide the first evidence that HTR4 expression is fine-tuned by miRNAs, and that this regulation is impaired either by the SNP c.∗61 T > C or by diminished levels of miR-16 and miR-103 suggesting that HTR4 might be involved in the development of IBS-D.

Original languageEnglish
Article number14680
Pages (from-to)1-14
Number of pages14
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 31 Oct 2017
Externally publishedYes

Bibliographical note

Copyright the Author(s) 2017. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

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