MIRAGE: the minimum information required for a glycomics experiment

William S. York, Sanjay Agravat, Kiyoko F. Aoki-Kinoshita, Ryan Mcbride, Matthew P. Campbell, Catherine E. Costello, Anne Dell, Ten Feizi, Stuart M. Haslam, Niclas Karlsson, Kay Hooi Khoo, Daniel Kolarich, Yan Liu, Milos Novotny, Nicolle H. Packer, James C. Paulson, Erdmann Rapp, Rene Ranzinger, Pauline M. Rudd, David F. SmithWeston B. Struwe, Michael Tiemeyer, Lance Wells, Joseph Zaia, Carsten Kettner*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    66 Citations (Scopus)
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    Abstract

    The MIRAGE (minimum information required for a glycomics experiment) initiative was founded in Seattle, WA, in November 2011 in order to develop guidelines for reporting the qualitative and quantitative results obtained by diverse types of glycomics analyses, including the conditions and techniques that were applied to prepare the glycans for analysis and generate the primary data along with the tools and parameters that were used to process and annotate this data. These guidelines must address a broad range of issues, as glycomics data are inherently complex and are generated using diverse methods, including mass spectrometry (MS), chromatography, glycan array-binding assays, nuclear magnetic resonance (NMR) and other rapidly developing technologies. The acceptance of these guidelines by scientists conducting research on biological systems in which glycans have a significant role will facilitate the evaluation and reproduction of glycomics experiments and data that is reported in scientific journals and uploaded to glycomics databases. As a first step, MIRAGE guidelines for glycan analysis by MS have been recently published (Kolarich D, Rapp E, Struwe WB, Haslam SM, Zaia J., et al. 2013. The minimum information required for a glycomics experiment (MIRAGE) project-Improving the standards for reporting mass spectrometry-based glycoanalytic data. Mol. Cell Proteomics. 12:991-995), allowing them to be implemented and evaluated in the context of real-world glycobiology research. In this paper, we set out the historical context, organization structure and overarching objectives of the MIRAGE initiative.

    Original languageEnglish
    Pages (from-to)402-406
    Number of pages5
    JournalGlycobiology
    Volume24
    Issue number5
    DOIs
    Publication statusPublished - May 2014

    Bibliographical note

    Copyright 2014 the author(s). First published in Glycobiology, 24(5), pp. 402-406. The original publication is available at http://www.doi.org/10.1093/glycob/cwu018, published by Oxford University Press. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

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