ML218 HCl is more efficient than capsaicin in inhibiting bacterial antigen-induced Cal 27 oral cancer cell proliferation

Rajdeep Chakraborty, Honghua Hu*, Charbel Darido, Karen Vickery, Shoba Ranganathan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

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Abstract

The bacterial antigen, lipopolysaccharide (LPS) and disruptions in calcium channels are independently known to influence oral cancer progression. Previously, we found that bacterial antigens, LPS and lipoteichoic acid (LTA) act as confounders during the action of capsaicin on Cal 27 oral cancer proliferation. As calcium channel drugs may affect oral cancer cell proliferation, we investigated the effect of ML218 HCl, a T-type voltage-gated calcium channel blocker, on the proliferation of Cal 27 oral cancer cells. We hypothesized that ML218 HCl could effectively reduce LPS-induced oral cancer cell proliferation. LPS and LTA antigens were added to Cal 27 oral cancer cells either prior to and/or concurrently with ML218 HCl treatment, and the efficacy of the treatment
was evaluated by measuring Cal 27 proliferation, cell death and apoptosis. ML218 HCl inhibited oral cancer cell proliferation, increased apoptosis and cell death, but their efficacy was significantly reduced in the presence of bacterial antigens. ML218 HCl proved more effective than capsaicin in reducing bacterial antigen-induced Cal 27 oral cancer cell proliferation. Our results also suggest an
interplay of proliferation factors during the bacterial antigens and calcium channel drug interaction in Cal 27. Bacterial antigen reduction of drug efficacy should be considered for developing newer pharmacological agents or testing the efficacy of the existing oral cancer chemotherapeutic agents. Finally, voltage gated calcium channel drugs should be considered for future oral cancer research.
Original languageEnglish
Article number12559
Pages (from-to)12559-12571
Number of pages12
JournalInternational Journal of Molecular Sciences
Volume22
Issue number22
DOIs
Publication statusPublished - 2 Nov 2021

Bibliographical note

Copyright the Author(s) 2021. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • lipopolysaccharide
  • lipoteichoic acid
  • oral cancer
  • ML218 HCl
  • capsaicin
  • proliferation
  • apoptosis
  • Oral cancer
  • Capsaicin
  • Lipoteichoic acid
  • Proliferation
  • Lipopolysaccharide
  • Apoptosis

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