Modulation of gene expression by Pseudomonas aeruginosa during chronic infection in the adult cystic fibrosis lung

Christopher Harmer*, Khaled Alnassafi, Honghuah Hu, Mark Elkins, Peter Bye, Barbara Rose, Stuart Cordwell, James A. Triccas, Colin Harbour, Jim Manos

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Chronic Pseudomonas aeruginosa infection is the leading cause of morbidity and mortality in cystic fibrosis (CF) patients. P. aeruginosa isolates undergo significant transcriptomic and proteomic modulation as they adapt to the niche environment of the CF lung and the host defences. This study characterized the in vitro virulence of isogenic strain pairs of P. aeruginosa epidemic or frequent clonal complexes (FCCs) and non-epidemic or infrequent clonal complexes (IFCCs) that were collected 5-8 years apart from five chronically infected adult CF patients. Strains showed a significant decrease in virulence over the course of chronic infection using a Caenorhabditis elegans slow-killing assay and in phenotypic tests for important virulence factors. This decrease in virulence correlated with numerous differentially expressed genes such as oprG, lasB, rsaL and lecB. Microarray analysis identified a large genomic island deletion in the IFCC strain pair that included type three secretion system effector and fimbrial subunit genes. This study presents novel in vitro data to examine the transcriptomic profiles of sequentially collected P. aeruginosa from CF adults. The genes with virulence-related functions identified here present potential targets for new therapies and vaccines against FCCs and IFCCs.

Original languageEnglish
Pages (from-to)2354-2363
Number of pages10
JournalMicrobiology (United Kingdom)
Volume159
DOIs
Publication statusPublished - Nov 2013
Externally publishedYes

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