Molecular analysis of the phospholipids of Escherichia coli K12

Michael Batley*, Nicolle H. Packer, John W. Redmond

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    32 Citations (Scopus)

    Abstract

    Phospholipids from Escherichia coli K12 were converted to 1,2-diacylglycerols with phospholipase C from Bacillus cereus. High-pressure liquid chromatography of 1,2-diacylglycerol p-methoxybenzoates on LiChrosorb RP-18 using 2-propanol/acetonitrile (35:65) as eluant permitted separation of 14 molecular species. The main combinations of fatty acids were 1-16:0-2-16:1, 1-16:0-2-cyclo-17:0 and 1-16:0-2-18:1. Positional isomers were not present. The l,2-di-16:0 compound was present at a significant level (7-10 mol%). Proportions of molecular species varied between phosphatidylethanolamine, phosphatidylglycerol and cardiolipin. Phospholipid from the outer membrane of E. coli K12 contained a lower level of molecules with two unsaturated chains than was present in the cytoplasmic membrane. The method is sensitive, has good resolving power and employs readily available equipment.

    Original languageEnglish
    Pages (from-to)400-405
    Number of pages6
    JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
    Volume710
    Issue number3
    DOIs
    Publication statusPublished - 12 Mar 1982

    Keywords

    • (E. coli)
    • HPLC
    • phospholipid analysis

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