TY - JOUR
T1 - Molecular basis of adaptive shift in body size in drosophila melanogaster
T2 - Functional and sequence analyses of the dca gene
AU - Lee, Siu F.
AU - Chen, Ying
AU - Varan, Aiden K.
AU - Wee, Choon W.
AU - Rako, Lea
AU - Axford, Jason K.
AU - Good, Robert T.
AU - Blacket, Mark J.
AU - Reuter, Caroline
AU - Partridge, Linda
AU - Hoffmann, Ary A.
PY - 2011/8
Y1 - 2011/8
N2 - Latitudinal body size clines in animals conforming to Bergmann's rule occur on many continents but isolating their underlying genetic basis remains a challenge. In Drosophila melanogaster, the gene Dca accounts for approximately 5-10% of the natural wing size variation (McKechnie SW, Blacket MJ, Song SV, Rako L, Carroll X, Johnson TK, Jensen LT, Lee SF, Wee CW, Hoffmann AA. 2010. A clinally varying promoter polymorphism associated with adaptive variation in wing size in Drosophila. Mol Ecol. 19:775-784). We present here functional evidence that Dca is a negative regulator of wing size. A significant negative latitudinal cline of Dca gene expression was detected in synchronized third instar larvae. In addition, we clarified the evolutionary history of the three most common Dca promoter alleles (Dca237-1, Dca237-2, and Dca247) and showed that the insertion allele (Dca247), whose frequency increases with latitude, is associated with larger wing centroid size and higher average cell number in male flies. Finally, we showed that the overall linkage disequilibrium (LD) was low in the Dca promoter and that the insertion/deletion polymorphism that defines the Dca alleles was in strong LD with two other upstream sites. Our results provide strong support that Dca is a candidate for climatic adaptation in D. melanogaster.
AB - Latitudinal body size clines in animals conforming to Bergmann's rule occur on many continents but isolating their underlying genetic basis remains a challenge. In Drosophila melanogaster, the gene Dca accounts for approximately 5-10% of the natural wing size variation (McKechnie SW, Blacket MJ, Song SV, Rako L, Carroll X, Johnson TK, Jensen LT, Lee SF, Wee CW, Hoffmann AA. 2010. A clinally varying promoter polymorphism associated with adaptive variation in wing size in Drosophila. Mol Ecol. 19:775-784). We present here functional evidence that Dca is a negative regulator of wing size. A significant negative latitudinal cline of Dca gene expression was detected in synchronized third instar larvae. In addition, we clarified the evolutionary history of the three most common Dca promoter alleles (Dca237-1, Dca237-2, and Dca247) and showed that the insertion allele (Dca247), whose frequency increases with latitude, is associated with larger wing centroid size and higher average cell number in male flies. Finally, we showed that the overall linkage disequilibrium (LD) was low in the Dca promoter and that the insertion/deletion polymorphism that defines the Dca alleles was in strong LD with two other upstream sites. Our results provide strong support that Dca is a candidate for climatic adaptation in D. melanogaster.
KW - adaptation
KW - body size
KW - cline
KW - Dca
KW - Drosophila melanogaster
KW - wing
UR - http://www.scopus.com/inward/record.url?scp=79960101012&partnerID=8YFLogxK
UR - http://purl.org/au-research/grants/arc/DP0770245
UR - http://purl.org/au-research/grants/arc/FL100100066
U2 - 10.1093/molbev/msr064
DO - 10.1093/molbev/msr064
M3 - Article
C2 - 21393605
AN - SCOPUS:79960101012
VL - 28
SP - 2393
EP - 2402
JO - Molecular Biology and Evolution
JF - Molecular Biology and Evolution
SN - 0737-4038
IS - 8
ER -