Molecular characterisation of the tammar wallaby (Macropus eugenii) CD3 epsilon chain cDNA

Julie M. Old, Elizabeth M. Deane, Gavan A. Harrison

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The cDNA encoding the epsilon chain of the tammar wallaby CD3 complex (CD3ε) was isolated by PCR. This is the first CD3 component to be cloned in a marsupial. The tammar wallaby cDNA coding region was 61.7 and 63.0% identical to the human and mouse cDNA coding sequences, respectively. Similarly, the predicted amino acid sequence was 56.5 and 52.9% identical to the human and mouse sequences. When compared with other known CD3ε peptide sequences, the most conserved region of the tammar wallaby CD3ε chain peptide was the cytoplasmic domain and the least conserved was the extracellular portion. Phylogenetic reconstruction based on the deduced amino acid sequence placed the tammar wallaby sequence in its expected position outside of all the eutherian mammals.

Original languageEnglish
Pages (from-to)359-364
Number of pages6
JournalMolecular Immunology
Volume38
Issue number5
DOIs
Publication statusPublished - 2001

Keywords

  • CD3ε
  • cDNA cloning
  • Evolution
  • Macropus eugenii
  • Marsupial

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