Molecular characterization of Eimeria species in macropods

Rongchang Yang; Stan Fenwick; Abbey Potter; Aileen Elliot; Michelle Power; Ian Beveridge; Una Ryan

doi:10.1016/j.exppara.2012.07.003

Molecular characterization of Eimeria species in macropods

Rongchang Yang, Stan Fenwick, Abbey Potter, Aileen Elliot, Michelle Power, Ian Beveridge, Una Ryan^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

A total of 597 faecal samples were collected from western grey kangaroos (Macropus fuliginosus), Euros (M. robustus), red kangaroos (M. rufus) in Western Australia and Eastern Grey Kangaroos (M. giganteus) from Victoria and screened for the presence of Eimeria by PCR at the 18S ribosomal RNA (rRNA) locus. The overall prevalence was 24.3% (145/597). At the 18S rRNA locus, sequences were obtained for 25 of the 145 positives. Phylogenetic analysis indicated that all the macropod-derived Eimeria species grouped in a separate marsupial clade that included Eimeria trichosuri from brushtail possums. At least 6 different clades were identified within the marsupial isolates and many of the genotypes identified are likely to be valid species, however morphological and biological data need to be collected to match sequences to previously characterized Eimeria species or identify if they are new species.

Original language	English
Pages (from-to)	216-221
Number of pages	6
Journal	Experimental Parasitology
Volume	132
Issue number	2
DOIs	https://doi.org/10.1016/j.exppara.2012.07.003
Publication status	Published - Oct 2012

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title = "Molecular characterization of Eimeria species in macropods",

abstract = "A total of 597 faecal samples were collected from western grey kangaroos (Macropus fuliginosus), Euros (M. robustus), red kangaroos (M. rufus) in Western Australia and Eastern Grey Kangaroos (M. giganteus) from Victoria and screened for the presence of Eimeria by PCR at the 18S ribosomal RNA (rRNA) locus. The overall prevalence was 24.3% (145/597). At the 18S rRNA locus, sequences were obtained for 25 of the 145 positives. Phylogenetic analysis indicated that all the macropod-derived Eimeria species grouped in a separate marsupial clade that included Eimeria trichosuri from brushtail possums. At least 6 different clades were identified within the marsupial isolates and many of the genotypes identified are likely to be valid species, however morphological and biological data need to be collected to match sequences to previously characterized Eimeria species or identify if they are new species.",

author = "Rongchang Yang and Stan Fenwick and Abbey Potter and Aileen Elliot and Michelle Power and Ian Beveridge and Una Ryan",

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T1 - Molecular characterization of Eimeria species in macropods

AU - Yang, Rongchang

AU - Fenwick, Stan

AU - Potter, Abbey

AU - Elliot, Aileen

AU - Power, Michelle

AU - Beveridge, Ian

AU - Ryan, Una

PY - 2012/10

Y1 - 2012/10

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AB - A total of 597 faecal samples were collected from western grey kangaroos (Macropus fuliginosus), Euros (M. robustus), red kangaroos (M. rufus) in Western Australia and Eastern Grey Kangaroos (M. giganteus) from Victoria and screened for the presence of Eimeria by PCR at the 18S ribosomal RNA (rRNA) locus. The overall prevalence was 24.3% (145/597). At the 18S rRNA locus, sequences were obtained for 25 of the 145 positives. Phylogenetic analysis indicated that all the macropod-derived Eimeria species grouped in a separate marsupial clade that included Eimeria trichosuri from brushtail possums. At least 6 different clades were identified within the marsupial isolates and many of the genotypes identified are likely to be valid species, however morphological and biological data need to be collected to match sequences to previously characterized Eimeria species or identify if they are new species.

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JO - Experimental Parasitology

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Molecular characterization of Eimeria species in macropods

Abstract

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