Molecular characterization of pig ST8Sia IV - A critical gene for the formation of neural cell adhesion molecule and its response to sialic acid supplement in piglets

Bing Wang, Honghua Hu, Bing Yu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

ST8Sia IV (polysialyltransferase IV gene) encodes a key enzyme that is required for polysialic acid synthesis. Polysialic acid is a component of the neural cell adhesion molecule and is necessary for synaptic plasticity of neural cells. We characterized 5.3 kb of pig ST8Sia IV cDNA and determined its expression profile in different organs. In hippocampus, ST8SiaIV mRNA levels were increased ̃4.5-fold in piglets with sialic acid as a milk supplement, which suggested that exogenous sialic acid is a conditionally essential nutrient for early brain development. Extensive analyses were also performed among its orthologs from human, mouse, rat, chicken, frog and zebrafish. Our results supported that the piglet is a better animal model than other nonprimate species in the studies of ST8Sia IV related metabolism and nutrition in human infants. This pig cDNA provides a basis for uncovering the roles of ST8Sia IV during piglet development and maturation.

Original languageEnglish
Pages (from-to)147-154
Number of pages8
JournalNutritional Neuroscience
Volume9
Issue number3-4
DOIs
Publication statusPublished - Jun 2006
Externally publishedYes

Keywords

  • Expression profile
  • Piglet
  • Polysialyltransferase
  • Sequencing
  • ST8Sia IV cDNA

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