Molecular evolution and antigenic variation of European brown hare syndrome virus (EBHSV)

Ana M. Lopes; Lorenzo Capucci; Dolores Gavier-Widén; Ghislaine Le Gall-Reculé; Emiliana Brocchi; Ilaria Barbieri; Agnès Quéméner; Jacques Le Pendu; Jemma L. Geoghegan; Edward C. Holmes; Pedro J. Esteves; Joana Abrantes

doi:10.1016/j.virol.2014.08.002

Molecular evolution and antigenic variation of European brown hare syndrome virus (EBHSV)

Ana M. Lopes, Lorenzo Capucci, Dolores Gavier-Widén, Ghislaine Le Gall-Reculé, Emiliana Brocchi, Ilaria Barbieri, Agnès Quéméner, Jacques Le Pendu, Jemma L. Geoghegan, Edward C. Holmes, Pedro J. Esteves, Joana Abrantes^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

European brown hare syndrome virus (EBHSV) is the aetiological agent of European brown hare syndrome (EBHS), a disease affecting Lepus europaeus and Lepus timidus first diagnosed in Sweden in 1980. To characterize EBHSV evolution we studied hare samples collected in Sweden between 1982 and 2008. Our molecular clock dating is compatible with EBHSV emergence in the 1970s. Phylogenetic analysis revealed two lineages: Group A persisted until 1989 when it apparently suffered extinction; Group B emerged in the mid-1980s and contains the most recent strains. Antigenic differences exist between groups, with loss of reactivity of some MAbs over time, which are associated with amino acid substitutions in recognized epitopes. A role for immune selection is also supported by the presence of positively selected codons in exposed regions of the capsid. Hence, EBHSV evolution is characterized by replacement of Group A by Group B viruses, suggesting that the latter possess a selective advantage.

Original language	English
Pages (from-to)	104-112
Number of pages	9
Journal	Virology
Volume	468-470
DOIs	https://doi.org/10.1016/j.virol.2014.08.002
Publication status	Published - Nov 2014
Externally published	Yes

Keywords

Antigenic variation
European brown hare syndrome virus (EBHSV)
Evolution

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title = "Molecular evolution and antigenic variation of European brown hare syndrome virus (EBHSV)",

abstract = "European brown hare syndrome virus (EBHSV) is the aetiological agent of European brown hare syndrome (EBHS), a disease affecting Lepus europaeus and Lepus timidus first diagnosed in Sweden in 1980. To characterize EBHSV evolution we studied hare samples collected in Sweden between 1982 and 2008. Our molecular clock dating is compatible with EBHSV emergence in the 1970s. Phylogenetic analysis revealed two lineages: Group A persisted until 1989 when it apparently suffered extinction; Group B emerged in the mid-1980s and contains the most recent strains. Antigenic differences exist between groups, with loss of reactivity of some MAbs over time, which are associated with amino acid substitutions in recognized epitopes. A role for immune selection is also supported by the presence of positively selected codons in exposed regions of the capsid. Hence, EBHSV evolution is characterized by replacement of Group A by Group B viruses, suggesting that the latter possess a selective advantage.",

keywords = "Antigenic variation, European brown hare syndrome virus (EBHSV), Evolution",

author = "Lopes, {Ana M.} and Lorenzo Capucci and Dolores Gavier-Wid{\'e}n and {Le Gall-Recul{\'e}}, Ghislaine and Emiliana Brocchi and Ilaria Barbieri and Agn{\`e}s Qu{\'e}m{\'e}ner and {Le Pendu}, Jacques and Geoghegan, {Jemma L.} and Holmes, {Edward C.} and Esteves, {Pedro J.} and Joana Abrantes",

year = "2014",

month = nov,

doi = "10.1016/j.virol.2014.08.002",

language = "English",

volume = "468-470",

pages = "104--112",

journal = "Virology",

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AU - Lopes, Ana M.

AU - Capucci, Lorenzo

AU - Gavier-Widén, Dolores

AU - Le Gall-Reculé, Ghislaine

AU - Brocchi, Emiliana

AU - Barbieri, Ilaria

AU - Quéméner, Agnès

AU - Le Pendu, Jacques

AU - Geoghegan, Jemma L.

AU - Holmes, Edward C.

AU - Esteves, Pedro J.

AU - Abrantes, Joana

PY - 2014/11

Y1 - 2014/11

N2 - European brown hare syndrome virus (EBHSV) is the aetiological agent of European brown hare syndrome (EBHS), a disease affecting Lepus europaeus and Lepus timidus first diagnosed in Sweden in 1980. To characterize EBHSV evolution we studied hare samples collected in Sweden between 1982 and 2008. Our molecular clock dating is compatible with EBHSV emergence in the 1970s. Phylogenetic analysis revealed two lineages: Group A persisted until 1989 when it apparently suffered extinction; Group B emerged in the mid-1980s and contains the most recent strains. Antigenic differences exist between groups, with loss of reactivity of some MAbs over time, which are associated with amino acid substitutions in recognized epitopes. A role for immune selection is also supported by the presence of positively selected codons in exposed regions of the capsid. Hence, EBHSV evolution is characterized by replacement of Group A by Group B viruses, suggesting that the latter possess a selective advantage.

AB - European brown hare syndrome virus (EBHSV) is the aetiological agent of European brown hare syndrome (EBHS), a disease affecting Lepus europaeus and Lepus timidus first diagnosed in Sweden in 1980. To characterize EBHSV evolution we studied hare samples collected in Sweden between 1982 and 2008. Our molecular clock dating is compatible with EBHSV emergence in the 1970s. Phylogenetic analysis revealed two lineages: Group A persisted until 1989 when it apparently suffered extinction; Group B emerged in the mid-1980s and contains the most recent strains. Antigenic differences exist between groups, with loss of reactivity of some MAbs over time, which are associated with amino acid substitutions in recognized epitopes. A role for immune selection is also supported by the presence of positively selected codons in exposed regions of the capsid. Hence, EBHSV evolution is characterized by replacement of Group A by Group B viruses, suggesting that the latter possess a selective advantage.

KW - Antigenic variation

KW - European brown hare syndrome virus (EBHSV)

KW - Evolution

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DO - 10.1016/j.virol.2014.08.002

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AN - SCOPUS:84906514013

VL - 468-470

SP - 104

EP - 112

JO - Virology

JF - Virology

SN - 0042-6822

ER -

Molecular evolution and antigenic variation of European brown hare syndrome virus (EBHSV)

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Keywords

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