TY - JOUR
T1 - Molecular imaging of inflammation in the ApoE -/- mouse model of atherosclerosis with IodoDPA
AU - Foss, Catherine A.
AU - Bedja, Djahida
AU - Mease, Ronnie C.
AU - Wang, Haofan
AU - Kass, David A.
AU - Chatterjee, Subroto
AU - Pomper, Martin G.
PY - 2015/5/22
Y1 - 2015/5/22
N2 - Background Atherosclerosis is a common and serious vascular disease predisposing individuals to myocardial infarction and stroke. Intravascular plaques, the pathologic lesions of atherosclerosis, are largely composed of cholesterol-laden luminal macrophage-rich infiltrates within a fibrous cap. The ability to detect those macrophages non-invasively within the aorta, carotid artery and other vessels would allow physicians to determine plaque burden, aiding management of patients with atherosclerosis. Methods and results We previously developed a low-molecular-weight imaging agent, [125I]iodo-DPA-713 (iodoDPA), which selectively targets macrophages. Here we use it to detect both intravascular macrophages and macrophage infiltrates within the myocardium in the ApoE -/- mouse model of atherosclerosis using single photon emission computed tomography (SPECT). SPECT data were confirmed by echocardiography, near-infrared fluorescence imaging and histology. SPECT images showed focal uptake of radiotracer at the aortic root in all ApoE -/- mice, while the age-matched controls were nearly devoid of radiotracer uptake. Focal radiotracer uptake along the descending aorta and within the myocardium was also observed in affected animals. Conclusions IodoDPA is a promising new imaging agent for atherosclerosis, with specificity for the macrophage component of the lesions involved.
AB - Background Atherosclerosis is a common and serious vascular disease predisposing individuals to myocardial infarction and stroke. Intravascular plaques, the pathologic lesions of atherosclerosis, are largely composed of cholesterol-laden luminal macrophage-rich infiltrates within a fibrous cap. The ability to detect those macrophages non-invasively within the aorta, carotid artery and other vessels would allow physicians to determine plaque burden, aiding management of patients with atherosclerosis. Methods and results We previously developed a low-molecular-weight imaging agent, [125I]iodo-DPA-713 (iodoDPA), which selectively targets macrophages. Here we use it to detect both intravascular macrophages and macrophage infiltrates within the myocardium in the ApoE -/- mouse model of atherosclerosis using single photon emission computed tomography (SPECT). SPECT data were confirmed by echocardiography, near-infrared fluorescence imaging and histology. SPECT images showed focal uptake of radiotracer at the aortic root in all ApoE -/- mice, while the age-matched controls were nearly devoid of radiotracer uptake. Focal radiotracer uptake along the descending aorta and within the myocardium was also observed in affected animals. Conclusions IodoDPA is a promising new imaging agent for atherosclerosis, with specificity for the macrophage component of the lesions involved.
UR - http://www.scopus.com/inward/record.url?scp=84937761251&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2015.03.171
DO - 10.1016/j.bbrc.2015.03.171
M3 - Article
C2 - 25858322
AN - SCOPUS:84937761251
VL - 461
SP - 70
EP - 75
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -