Molecular Imprint for Electrochemical Detection of Streptomycin Residues Using Enzyme Signal Amplification

Xiaohua Que, Bingqian Liu, Libing Fu, Junyang Zhuang, Guonan Chen, Dianping Tang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

We have designed a new molecularly imprinted co-polymer (MIP) for the sensitive detection of streptomycin (STR) in food using enzymes as signal amplification. The MIP was fabricated via co-polymerization of aniline and o-phenylenediamine on gold substrate in the presence of STR as template. The assay is based on competitive binding of free STR and glucose oxidase-labeled STR (GOx-STR) to the imprinters on the MIP. On addition of glucose, hydrogen peroxide is formed that is detected by differential pulse voltammetry. Under optimal conditions, the decrease of the catalytic current is proportional to the STR concentration in the range from 0.01 to 10ngmL-1, with a detection limit (LOD) of 7.0pgmL-1 STR (at 3sB). Intra- and inter-assay coefficients of variation (CVs) are<10.5%. The system was further validated and evaluated with STR-spiked samples including honey and milk, and the recovery was between 82 and 124.2%.

Original languageEnglish
Pages (from-to)531-537
Number of pages7
JournalElectroanalysis
Volume25
Issue number2
DOIs
Publication statusPublished - Feb 2013
Externally publishedYes

Keywords

  • Electrochemistry
  • Enzymatic catalysis
  • Molecularly imprinted polymers
  • Signal amplification
  • Streptomycin residue

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