Molecular serogrouping of porcine enterotoxigenic Escherichia coli from Australia

Sam Abraham; James Chin; Huub J. M. Brouwers; Ren Zhang; Toni A. Chapman

doi:10.1016/j.mimet.2011.10.016

Molecular serogrouping of porcine enterotoxigenic Escherichia coli from Australia

Sam Abraham, James Chin, Huub J. M. Brouwers, Ren Zhang, Toni A. Chapman^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Enterotoxigenic Escherichia coli (ETEC) is a common etiological agent of neonatal, pre and post weaning diarrhoea in piglets. One of the most important steps in the diagnosis and epidemiological understanding of this organism is accurate serogrouping. In many instances, however, conventional serogrouping fails to produce accurate identification of serogroups. In this communication we report a modified and simplified molecular serogrouping method (rfb-RFLP) for the accurate identification of the most common porcine ETEC strains that cause neonatal, pre and post weaning diarrhoea in Australia. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	73-76
Number of pages	4
Journal	Journal of Microbiological Methods
Volume	88
Issue number	1
DOIs	https://doi.org/10.1016/j.mimet.2011.10.016
Publication status	Published - Jan 2012
Externally published	Yes

Keywords

Serogrouping
Molecular serogrouping
Enterotoxigenic E. coli
Neonatal diarrhoea
Post-weaning diarrhoea
Diagnosis
ANTIGEN GENE-CLUSTER
VIRULENCE GENES
IDENTIFICATION
PIGS
RESTRICTION
SWINE

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10.1016/j.mimet.2011.10.016

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title = "Molecular serogrouping of porcine enterotoxigenic Escherichia coli from Australia",

abstract = "Enterotoxigenic Escherichia coli (ETEC) is a common etiological agent of neonatal, pre and post weaning diarrhoea in piglets. One of the most important steps in the diagnosis and epidemiological understanding of this organism is accurate serogrouping. In many instances, however, conventional serogrouping fails to produce accurate identification of serogroups. In this communication we report a modified and simplified molecular serogrouping method (rfb-RFLP) for the accurate identification of the most common porcine ETEC strains that cause neonatal, pre and post weaning diarrhoea in Australia. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.",

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author = "Sam Abraham and James Chin and Brouwers, {Huub J. M.} and Ren Zhang and Chapman, {Toni A.}",

year = "2012",

month = jan,

doi = "10.1016/j.mimet.2011.10.016",

language = "English",

volume = "88",

pages = "73--76",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Molecular serogrouping of porcine enterotoxigenic Escherichia coli from Australia

AU - Abraham, Sam

AU - Chin, James

AU - Brouwers, Huub J. M.

AU - Zhang, Ren

AU - Chapman, Toni A.

PY - 2012/1

Y1 - 2012/1

N2 - Enterotoxigenic Escherichia coli (ETEC) is a common etiological agent of neonatal, pre and post weaning diarrhoea in piglets. One of the most important steps in the diagnosis and epidemiological understanding of this organism is accurate serogrouping. In many instances, however, conventional serogrouping fails to produce accurate identification of serogroups. In this communication we report a modified and simplified molecular serogrouping method (rfb-RFLP) for the accurate identification of the most common porcine ETEC strains that cause neonatal, pre and post weaning diarrhoea in Australia. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.

AB - Enterotoxigenic Escherichia coli (ETEC) is a common etiological agent of neonatal, pre and post weaning diarrhoea in piglets. One of the most important steps in the diagnosis and epidemiological understanding of this organism is accurate serogrouping. In many instances, however, conventional serogrouping fails to produce accurate identification of serogroups. In this communication we report a modified and simplified molecular serogrouping method (rfb-RFLP) for the accurate identification of the most common porcine ETEC strains that cause neonatal, pre and post weaning diarrhoea in Australia. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.

KW - Serogrouping

KW - Molecular serogrouping

KW - Enterotoxigenic E. coli

KW - Neonatal diarrhoea

KW - Post-weaning diarrhoea

KW - Diagnosis

KW - ANTIGEN GENE-CLUSTER

KW - VIRULENCE GENES

KW - IDENTIFICATION

KW - PIGS

KW - RESTRICTION

KW - SWINE

U2 - 10.1016/j.mimet.2011.10.016

DO - 10.1016/j.mimet.2011.10.016

M3 - Article

C2 - 22093999

SN - 0167-7012

VL - 88

SP - 73

EP - 76

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 1

ER -

Molecular serogrouping of porcine enterotoxigenic Escherichia coli from Australia

Abstract

Keywords

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