Molecular serogrouping of porcine enterotoxigenic Escherichia coli from Australia

Sam Abraham, James Chin, Huub J. M. Brouwers, Ren Zhang, Toni A. Chapman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Enterotoxigenic Escherichia coli (ETEC) is a common etiological agent of neonatal, pre and post weaning diarrhoea in piglets. One of the most important steps in the diagnosis and epidemiological understanding of this organism is accurate serogrouping. In many instances, however, conventional serogrouping fails to produce accurate identification of serogroups. In this communication we report a modified and simplified molecular serogrouping method (rfb-RFLP) for the accurate identification of the most common porcine ETEC strains that cause neonatal, pre and post weaning diarrhoea in Australia. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)73-76
Number of pages4
JournalJournal of Microbiological Methods
Volume88
Issue number1
DOIs
Publication statusPublished - Jan 2012
Externally publishedYes

Keywords

  • Serogrouping
  • Molecular serogrouping
  • Enterotoxigenic E. coli
  • Neonatal diarrhoea
  • Post-weaning diarrhoea
  • Diagnosis
  • ANTIGEN GENE-CLUSTER
  • VIRULENCE GENES
  • IDENTIFICATION
  • PIGS
  • RESTRICTION
  • SWINE

Cite this