Molecular variation of meningococcal serotype 4 antigen genes

Rachel Urwin; Ian M. Feavers; Dennis M. Jones; Martin C. J. Maiden; Andrew J. Fox

doi:10.1017/s0950268898008942

Molecular variation of meningococcal serotype 4 antigen genes

Rachel Urwin, Ian M. Feavers, Dennis M. Jones, Martin C. J. Maiden, Andrew J. Fox

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

Changes in the frequency of serogroup B non serotypable (B[ratio ]NT) meningococci isolated in England and Wales were investigated by T-track fingerprint analysis, DNA nucleotide sequence determination, and serotyping by whole cell ELISA and dot blot assay. Seventy-three per cent of the isolates designated as B[ratio ]NT by the Meningococcal Reference Unit (MRU) dot blot assay during 1993–4, expressed variants of the serotyping antigen, PorB, that were serotype 4 by whole cell ELISA. T-track fingerprint patterns of these and other ‘serotype 4’ isolates revealed five distinct porB alleles which were shown by nucleotide sequence determination to encode different peptide sequences. Differential binding of the ‘serotype 4’ mAbs MN14G21 and 5DC4C8G8 in whole cell ELISA and dot blot assays was the result, (i) of differences in the peptide sequence of predicted surface loop I and (ii) an amino acid deletion in predicted loop VI of the PorB protein.

Original language	English
Pages (from-to)	95-101
Number of pages	7
Journal	Epidemiology and Infection
Volume	121
Issue number	1
DOIs	https://doi.org/10.1017/s0950268898008942
Publication status	Published - Aug 1998
Externally published	Yes

Access to Document

10.1017/s0950268898008942

Cite this

@article{20b0acdb0106450799eeafe7d4500b91,

title = "Molecular variation of meningococcal serotype 4 antigen genes",

abstract = "Changes in the frequency of serogroup B non serotypable (B[ratio ]NT) meningococci isolated in England and Wales were investigated by T-track fingerprint analysis, DNA nucleotide sequence determination, and serotyping by whole cell ELISA and dot blot assay. Seventy-three per cent of the isolates designated as B[ratio ]NT by the Meningococcal Reference Unit (MRU) dot blot assay during 1993–4, expressed variants of the serotyping antigen, PorB, that were serotype 4 by whole cell ELISA. T-track fingerprint patterns of these and other {\textquoteleft}serotype 4{\textquoteright} isolates revealed five distinct porB alleles which were shown by nucleotide sequence determination to encode different peptide sequences. Differential binding of the {\textquoteleft}serotype 4{\textquoteright} mAbs MN14G21 and 5DC4C8G8 in whole cell ELISA and dot blot assays was the result, (i) of differences in the peptide sequence of predicted surface loop I and (ii) an amino acid deletion in predicted loop VI of the PorB protein.",

author = "Rachel Urwin and Feavers, {Ian M.} and Jones, {Dennis M.} and Maiden, {Martin C. J.} and Fox, {Andrew J.}",

year = "1998",

month = aug,

doi = "10.1017/s0950268898008942",

language = "English",

volume = "121",

pages = "95--101",

journal = "Epidemiology and Infection",

issn = "0950-2688",

publisher = "Cambridge University Press (CUP)",

number = "1",

}

TY - JOUR

T1 - Molecular variation of meningococcal serotype 4 antigen genes

AU - Urwin, Rachel

AU - Feavers, Ian M.

AU - Jones, Dennis M.

AU - Maiden, Martin C. J.

AU - Fox, Andrew J.

PY - 1998/8

Y1 - 1998/8

N2 - Changes in the frequency of serogroup B non serotypable (B[ratio ]NT) meningococci isolated in England and Wales were investigated by T-track fingerprint analysis, DNA nucleotide sequence determination, and serotyping by whole cell ELISA and dot blot assay. Seventy-three per cent of the isolates designated as B[ratio ]NT by the Meningococcal Reference Unit (MRU) dot blot assay during 1993–4, expressed variants of the serotyping antigen, PorB, that were serotype 4 by whole cell ELISA. T-track fingerprint patterns of these and other ‘serotype 4’ isolates revealed five distinct porB alleles which were shown by nucleotide sequence determination to encode different peptide sequences. Differential binding of the ‘serotype 4’ mAbs MN14G21 and 5DC4C8G8 in whole cell ELISA and dot blot assays was the result, (i) of differences in the peptide sequence of predicted surface loop I and (ii) an amino acid deletion in predicted loop VI of the PorB protein.

AB - Changes in the frequency of serogroup B non serotypable (B[ratio ]NT) meningococci isolated in England and Wales were investigated by T-track fingerprint analysis, DNA nucleotide sequence determination, and serotyping by whole cell ELISA and dot blot assay. Seventy-three per cent of the isolates designated as B[ratio ]NT by the Meningococcal Reference Unit (MRU) dot blot assay during 1993–4, expressed variants of the serotyping antigen, PorB, that were serotype 4 by whole cell ELISA. T-track fingerprint patterns of these and other ‘serotype 4’ isolates revealed five distinct porB alleles which were shown by nucleotide sequence determination to encode different peptide sequences. Differential binding of the ‘serotype 4’ mAbs MN14G21 and 5DC4C8G8 in whole cell ELISA and dot blot assays was the result, (i) of differences in the peptide sequence of predicted surface loop I and (ii) an amino acid deletion in predicted loop VI of the PorB protein.

U2 - 10.1017/s0950268898008942

DO - 10.1017/s0950268898008942

M3 - Article

C2 - 9747760

SN - 0950-2688

VL - 121

SP - 95

EP - 101

JO - Epidemiology and Infection

JF - Epidemiology and Infection

IS - 1

ER -

Molecular variation of meningococcal serotype 4 antigen genes

Abstract

Access to Document

Fingerprint

Cite this