Monitoring oncogenic B-RAF-induced senescence in melanocytes

Sieu L. Tran; Helen Rizos

doi:10.1007/978-1-62703-239-1_21

Monitoring oncogenic B-RAF-induced senescence in melanocytes

Sieu L. Tran, Helen Rizos

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Citation (Scopus)

Abstract

The B-RAF kinase is a downstream effector of the RAS family of proto-oncogenes and is constitutively activated in the majority of human melanomas. The common oncogenic B-RAF^V600E mutant cooperates with additional genetic lesions to transform immortal murine and human cells. In primary cells, however, B-RAF^V600E triggers a rapid cell cycle arrest that is phenotypically indistinguishable from cellular senescence. Here we describe the analyses of B-RAF-induced senescence in primary human melanocytes using recombinant lentiviruses.

Original language	English
Title of host publication	Cell Senescence
Subtitle of host publication	Methods and Protocols
Editors	Lorenzo Galluzzi, Ilio Vitale, Oliver Kepp, Guido Kroemer
Place of Publication	New York
Publisher	Humana Press Inc.
Pages	313-326
Number of pages	14
ISBN (Electronic)	9781627032391
ISBN (Print)	9781627032384
DOIs	https://doi.org/10.1007/978-1-62703-239-1_21
Publication status	Published - 2013
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Publisher	Humana Press
Volume	965
ISSN (Print)	1064-3745

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10.1007/978-1-62703-239-1_21

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title = "Monitoring oncogenic B-RAF-induced senescence in melanocytes",

abstract = "The B-RAF kinase is a downstream effector of the RAS family of proto-oncogenes and is constitutively activated in the majority of human melanomas. The common oncogenic B-RAFV600E mutant cooperates with additional genetic lesions to transform immortal murine and human cells. In primary cells, however, B-RAFV600E triggers a rapid cell cycle arrest that is phenotypically indistinguishable from cellular senescence. Here we describe the analyses of B-RAF-induced senescence in primary human melanocytes using recombinant lentiviruses.",

author = "Tran, {Sieu L.} and Helen Rizos",

year = "2013",

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Monitoring oncogenic B-RAF-induced senescence in melanocytes. / Tran, Sieu L.; Rizos, Helen.
Cell Senescence: Methods and Protocols. ed. / Lorenzo Galluzzi; Ilio Vitale; Oliver Kepp; Guido Kroemer. New York: Humana Press Inc., 2013. p. 313-326 (Methods in Molecular Biology; Vol. 965).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

TY - CHAP

T1 - Monitoring oncogenic B-RAF-induced senescence in melanocytes

AU - Tran, Sieu L.

AU - Rizos, Helen

PY - 2013

Y1 - 2013

N2 - The B-RAF kinase is a downstream effector of the RAS family of proto-oncogenes and is constitutively activated in the majority of human melanomas. The common oncogenic B-RAFV600E mutant cooperates with additional genetic lesions to transform immortal murine and human cells. In primary cells, however, B-RAFV600E triggers a rapid cell cycle arrest that is phenotypically indistinguishable from cellular senescence. Here we describe the analyses of B-RAF-induced senescence in primary human melanocytes using recombinant lentiviruses.

AB - The B-RAF kinase is a downstream effector of the RAS family of proto-oncogenes and is constitutively activated in the majority of human melanomas. The common oncogenic B-RAFV600E mutant cooperates with additional genetic lesions to transform immortal murine and human cells. In primary cells, however, B-RAFV600E triggers a rapid cell cycle arrest that is phenotypically indistinguishable from cellular senescence. Here we describe the analyses of B-RAF-induced senescence in primary human melanocytes using recombinant lentiviruses.

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DO - 10.1007/978-1-62703-239-1_21

M3 - Chapter

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AN - SCOPUS:84878873782

SN - 9781627032384

T3 - Methods in Molecular Biology

SP - 313

EP - 326

BT - Cell Senescence

A2 - Galluzzi, Lorenzo

A2 - Vitale, Ilio

A2 - Kepp, Oliver

A2 - Kroemer, Guido

PB - Humana Press Inc.

CY - New York

ER -

Monitoring oncogenic B-RAF-induced senescence in melanocytes

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