Monitoring oncogenic B-RAF-induced senescence in melanocytes

Sieu L. Tran, Helen Rizos

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)


The B-RAF kinase is a downstream effector of the RAS family of proto-oncogenes and is constitutively activated in the majority of human melanomas. The common oncogenic B-RAFV600E mutant cooperates with additional genetic lesions to transform immortal murine and human cells. In primary cells, however, B-RAFV600E triggers a rapid cell cycle arrest that is phenotypically indistinguishable from cellular senescence. Here we describe the analyses of B-RAF-induced senescence in primary human melanocytes using recombinant lentiviruses.

Original languageEnglish
Title of host publicationCell Senescence
Subtitle of host publicationMethods and Protocols
EditorsLorenzo Galluzzi, Ilio Vitale, Oliver Kepp, Guido Kroemer
Place of PublicationNew York
PublisherHumana Press Inc.
Number of pages14
ISBN (Electronic)9781627032391
ISBN (Print)9781627032384
Publication statusPublished - 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
ISSN (Print)1064-3745

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