Abstract
The hemoprotein indoleamine 2,3-dioxygenase (IDO) is the first and rate-limiting enzyme in the most significant pathway for mammalian tryptophan metabolism. It has received considerable attention in recent years, particularly due to its dual role in immunity and the pathogenesis of many diseases. Reported here are differences and similarities between biochemical behaviour and structural features of recombinant human IDO and recombinant mouse IDO. Significant differences were observed in the conversion of substrates and pH stability. Differences in inhibitor potency and thermal stability were also noted. Secondary structural features were broadly similar but variation between species was apparent, particularly in the α-helix portion of the enzymes. With mouse models substituting for human diseases, the differences between mouse and human IDO must be recognised before applying experimental findings from one system to the next.
| Language | English |
|---|---|
| Pages | 99-106 |
| Number of pages | 8 |
| Journal | Amino Acids |
| Volume | 36 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2009 |
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Mouse and human indoleamine 2,3-dioxygenase display some distinct biochemical and structural properties. / Austin, Christopher J D; Astelbauer, Florian; Kosim-Satyaputra, Priambudi; Ball, Helen J.; Willows, Robert D.; Jamie, Joanne F.; Hunt, Nicholas H.
In: Amino Acids, Vol. 36, No. 1, 01.2009, p. 99-106.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Mouse and human indoleamine 2,3-dioxygenase display some distinct biochemical and structural properties
AU - Austin, Christopher J D
AU - Astelbauer, Florian
AU - Kosim-Satyaputra, Priambudi
AU - Ball, Helen J.
AU - Willows, Robert D.
AU - Jamie, Joanne F.
AU - Hunt, Nicholas H.
PY - 2009/1
Y1 - 2009/1
N2 - The hemoprotein indoleamine 2,3-dioxygenase (IDO) is the first and rate-limiting enzyme in the most significant pathway for mammalian tryptophan metabolism. It has received considerable attention in recent years, particularly due to its dual role in immunity and the pathogenesis of many diseases. Reported here are differences and similarities between biochemical behaviour and structural features of recombinant human IDO and recombinant mouse IDO. Significant differences were observed in the conversion of substrates and pH stability. Differences in inhibitor potency and thermal stability were also noted. Secondary structural features were broadly similar but variation between species was apparent, particularly in the α-helix portion of the enzymes. With mouse models substituting for human diseases, the differences between mouse and human IDO must be recognised before applying experimental findings from one system to the next.
AB - The hemoprotein indoleamine 2,3-dioxygenase (IDO) is the first and rate-limiting enzyme in the most significant pathway for mammalian tryptophan metabolism. It has received considerable attention in recent years, particularly due to its dual role in immunity and the pathogenesis of many diseases. Reported here are differences and similarities between biochemical behaviour and structural features of recombinant human IDO and recombinant mouse IDO. Significant differences were observed in the conversion of substrates and pH stability. Differences in inhibitor potency and thermal stability were also noted. Secondary structural features were broadly similar but variation between species was apparent, particularly in the α-helix portion of the enzymes. With mouse models substituting for human diseases, the differences between mouse and human IDO must be recognised before applying experimental findings from one system to the next.
UR - http://www.scopus.com/inward/record.url?scp=58149252435&partnerID=8YFLogxK
U2 - 10.1007/s00726-008-0037-6
DO - 10.1007/s00726-008-0037-6
M3 - Article
VL - 36
SP - 99
EP - 106
JO - Amino Acids
T2 - Amino Acids
JF - Amino Acids
SN - 0939-4451
IS - 1
ER -