Mouse and human indoleamine 2,3-dioxygenase display some distinct biochemical and structural properties

Christopher J D Austin, Florian Astelbauer, Priambudi Kosim-Satyaputra, Helen J. Ball, Robert D. Willows, Joanne F. Jamie, Nicholas H. Hunt

    Research output: Contribution to journalArticlepeer-review

    31 Citations (Scopus)

    Abstract

    The hemoprotein indoleamine 2,3-dioxygenase (IDO) is the first and rate-limiting enzyme in the most significant pathway for mammalian tryptophan metabolism. It has received considerable attention in recent years, particularly due to its dual role in immunity and the pathogenesis of many diseases. Reported here are differences and similarities between biochemical behaviour and structural features of recombinant human IDO and recombinant mouse IDO. Significant differences were observed in the conversion of substrates and pH stability. Differences in inhibitor potency and thermal stability were also noted. Secondary structural features were broadly similar but variation between species was apparent, particularly in the α-helix portion of the enzymes. With mouse models substituting for human diseases, the differences between mouse and human IDO must be recognised before applying experimental findings from one system to the next.

    Original languageEnglish
    Pages (from-to)99-106
    Number of pages8
    JournalAmino Acids
    Volume36
    Issue number1
    DOIs
    Publication statusPublished - Jan 2009

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