Mouse and human indoleamine 2,3-dioxygenase display some distinct biochemical and structural properties

Christopher J D Austin, Florian Astelbauer, Priambudi Kosim-Satyaputra, Helen J. Ball, Robert D. Willows, Joanne F. Jamie, Nicholas H. Hunt

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The hemoprotein indoleamine 2,3-dioxygenase (IDO) is the first and rate-limiting enzyme in the most significant pathway for mammalian tryptophan metabolism. It has received considerable attention in recent years, particularly due to its dual role in immunity and the pathogenesis of many diseases. Reported here are differences and similarities between biochemical behaviour and structural features of recombinant human IDO and recombinant mouse IDO. Significant differences were observed in the conversion of substrates and pH stability. Differences in inhibitor potency and thermal stability were also noted. Secondary structural features were broadly similar but variation between species was apparent, particularly in the α-helix portion of the enzymes. With mouse models substituting for human diseases, the differences between mouse and human IDO must be recognised before applying experimental findings from one system to the next.

LanguageEnglish
Pages99-106
Number of pages8
JournalAmino Acids
Volume36
Issue number1
DOIs
Publication statusPublished - Jan 2009

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Indoleamine-Pyrrole 2,3,-Dioxygenase
Structural properties
Enzymes
Metabolism
Tryptophan
Immunity
Thermodynamic stability
Hot Temperature
Substrates

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Austin, Christopher J D ; Astelbauer, Florian ; Kosim-Satyaputra, Priambudi ; Ball, Helen J. ; Willows, Robert D. ; Jamie, Joanne F. ; Hunt, Nicholas H. / Mouse and human indoleamine 2,3-dioxygenase display some distinct biochemical and structural properties. In: Amino Acids. 2009 ; Vol. 36, No. 1. pp. 99-106.
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Mouse and human indoleamine 2,3-dioxygenase display some distinct biochemical and structural properties. / Austin, Christopher J D; Astelbauer, Florian; Kosim-Satyaputra, Priambudi; Ball, Helen J.; Willows, Robert D.; Jamie, Joanne F.; Hunt, Nicholas H.

In: Amino Acids, Vol. 36, No. 1, 01.2009, p. 99-106.

Research output: Contribution to journalArticleResearchpeer-review

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