Multi-locus genotyping of stored sperm reveals female remating rates in wild populations of the Queensland fruit fly

Jason Shadmany; Phillip W. Taylor; Heng Lin Yeap; Siu Fai Lee

doi:10.1016/j.cris.2022.100040

Multi-locus genotyping of stored sperm reveals female remating rates in wild populations of the Queensland fruit fly

Jason Shadmany, Phillip W. Taylor, Heng Lin Yeap, Siu Fai Lee^*

^*Corresponding author for this work

Applied BioSciences

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Abstract

Female insects commonly have more than one mate during a breeding period ('polyandry'), storing and using sperm from multiple males. In addition to its evolutionary significance, insect polyandry has practical implications for pest management that relies on the sterile insect technique (SIT). The Queensland fruit fly, Bactrocera tryoni (Froggatt), is a major horticultural pest in Australia, and outbreaks are managed by SIT in some regions. The present study provides the first evidence for polyandry in female B. tryoni from field populations from New South Wales (NSW) and Queensland (QLD) through multi-locus genotyping (ten microsatellite markers in four fluorescent multiplexes) of the stored sperm in ovipositing females. Polyandry level was significantly higher in the NSW collection (80.0 %) than the QLD collection (26.1 %), suggesting substantial regional and/or temporal variation. These findings have important implications for the use of SIT to suppress B. tryoni populations and to eradicate outbreaks.

Original language	English
Article number	100040
Pages (from-to)	1-6
Number of pages	6
Journal	Current Research in Insect Science
Volume	2
Early online date	7 Jun 2022
DOIs	https://doi.org/10.1016/j.cris.2022.100040
Publication status	Published - 2022

Bibliographical note

Copyright the Author(s) 2022. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

Bactrocera tryoni
microsatellites
Polyandry
sperm genotyping
sterile insect technique

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10.1016/j.cris.2022.100040

Publisher version (open access)Final published version, 751 KBLicence: CC BY-NC-ND

Cite this

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title = "Multi-locus genotyping of stored sperm reveals female remating rates in wild populations of the Queensland fruit fly",

abstract = "Female insects commonly have more than one mate during a breeding period ('polyandry'), storing and using sperm from multiple males. In addition to its evolutionary significance, insect polyandry has practical implications for pest management that relies on the sterile insect technique (SIT). The Queensland fruit fly, Bactrocera tryoni (Froggatt), is a major horticultural pest in Australia, and outbreaks are managed by SIT in some regions. The present study provides the first evidence for polyandry in female B. tryoni from field populations from New South Wales (NSW) and Queensland (QLD) through multi-locus genotyping (ten microsatellite markers in four fluorescent multiplexes) of the stored sperm in ovipositing females. Polyandry level was significantly higher in the NSW collection (80.0 %) than the QLD collection (26.1 %), suggesting substantial regional and/or temporal variation. These findings have important implications for the use of SIT to suppress B. tryoni populations and to eradicate outbreaks.",

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author = "Jason Shadmany and Taylor, {Phillip W.} and Yeap, {Heng Lin} and Lee, {Siu Fai}",

note = "Copyright the Author(s) 2022. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher. ",

year = "2022",

doi = "10.1016/j.cris.2022.100040",

language = "English",

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AU - Lee, Siu Fai

N1 - Copyright the Author(s) 2022. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2022

Y1 - 2022

N2 - Female insects commonly have more than one mate during a breeding period ('polyandry'), storing and using sperm from multiple males. In addition to its evolutionary significance, insect polyandry has practical implications for pest management that relies on the sterile insect technique (SIT). The Queensland fruit fly, Bactrocera tryoni (Froggatt), is a major horticultural pest in Australia, and outbreaks are managed by SIT in some regions. The present study provides the first evidence for polyandry in female B. tryoni from field populations from New South Wales (NSW) and Queensland (QLD) through multi-locus genotyping (ten microsatellite markers in four fluorescent multiplexes) of the stored sperm in ovipositing females. Polyandry level was significantly higher in the NSW collection (80.0 %) than the QLD collection (26.1 %), suggesting substantial regional and/or temporal variation. These findings have important implications for the use of SIT to suppress B. tryoni populations and to eradicate outbreaks.

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Multi-locus genotyping of stored sperm reveals female remating rates in wild populations of the Queensland fruit fly

Abstract

Bibliographical note

Keywords

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Raising Qfly Sterile Insect Technique to World Standard

Cite this

Multi-locus genotyping of stored sperm reveals female remating rates in wild populations of the Queensland fruit fly

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Projects

Raising Qfly Sterile Insect Technique to World Standard

Cite this