Multi-locus sequence analysis, taxonomic resolution and biogeography of marine Synechococcus

Sophie Mazard, Martin Ostrowski, Frédéric Partensky, David J. Scanlan

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Summary: Conserved markers such as the 16S rRNA gene do not provide sufficient molecular resolution to identify spatially structured populations of marine Synechococcus, or 'ecotypes' adapted to distinct ecological niches. Multi-locus sequence analysis targeting seven 'core' genes was employed to taxonomically resolve Synechococcus isolates and correlate previous phylogenetic analyses encompassing a range of markers. Despite the recognized importance of lateral gene transfer in shaping the genomes of marine cyanobacteria, multi-locus sequence analysis of more than 120 isolates reflects a clonal population structure of major lineages and subgroups. A single core genome locus, petB, encoding the cytochrome b 6 subunit of the cytochrome b 6f complex, was selected to expand our understanding of the diversity and ecology of marine Synechococcus populations. Environmental petB sequences cloned from contrasting sites highlight numerous genetically and ecologically distinct clusters, some of which represent novel, environmentally abundant clades without cultured representatives. With a view to scaling ecological analyses, the short sequence, taxonomic resolution and accurate automated alignment of petB is ideally suited to high-throughput and high-resolution sequencing projects to explore links between the ecology, evolution and biology of marine Synechococcus.

LanguageEnglish
Pages372-386
Number of pages15
JournalEnvironmental microbiology
Volume14
Issue number2
DOIs
Publication statusPublished - Feb 2012
Externally publishedYes

Fingerprint

Synechococcus
biogeography
Sequence Analysis
Marine Biology
Cytochromes b
cytochrome
genome
cytochrome b
ecology
gene
gene transfer
ecotype
Genome
Population
Ecotype
Horizontal Gene Transfer
targeting
marine science
population structure
cyanobacterium

Cite this

Mazard, Sophie ; Ostrowski, Martin ; Partensky, Frédéric ; Scanlan, David J. / Multi-locus sequence analysis, taxonomic resolution and biogeography of marine Synechococcus. In: Environmental microbiology. 2012 ; Vol. 14, No. 2. pp. 372-386.
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Multi-locus sequence analysis, taxonomic resolution and biogeography of marine Synechococcus. / Mazard, Sophie; Ostrowski, Martin; Partensky, Frédéric; Scanlan, David J.

In: Environmental microbiology, Vol. 14, No. 2, 02.2012, p. 372-386.

Research output: Contribution to journalArticleResearchpeer-review

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