Multiple positive and negative cis-acting elements of the STA2 gene regulate glucoamylase synthesis in Saccharomyces cerevisiae

Marius G. Lambrechts; Isak S. Pretorius; Vincenzo S. D'Aguanno; Paul Sollitti; Julius Marmur

doi:10.1016/0378-1119(94)90286-0

Multiple positive and negative cis-acting elements of the STA2 gene regulate glucoamylase synthesis in Saccharomyces cerevisiae

Marius G. Lambrechts, Isak S. Pretorius^*, Vincenzo S. D'Aguanno, Paul Sollitti, Julius Marmur

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Expression of the glucoamylase-encoding gene (STA2) in Saccharomyces cerevisiae was previously shown to be regulated transcriptionally by both positive and negative factors. The objective of this work was to identify the cis-acting elements responsible for STA2 transcriptional activation as well as the transcriptional repressor effects of STA10 and MATa/MATα. We identified two upstream activation regions (UAS). Three repressor regions responsive to STA10-mediated repression were identified, as well as two regions for down-regulation of STA2 expression. MATa/MATα repression appears to effect STA2 expression either downstream from the translational start site or, indirectly, since no functional al/α2-responsive sequence was identified in the promoter region.

Original language	English
Pages (from-to)	137-144
Number of pages	8
Journal	Gene
Volume	146
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(94)90286-0
Publication status	Published - 2 Sept 1994
Externally published	Yes

Access to Document

10.1016/0378-1119(94)90286-0

Cite this

@article{c9e16b231fe547f697eee9d5146c5003,

title = "Multiple positive and negative cis-acting elements of the STA2 gene regulate glucoamylase synthesis in Saccharomyces cerevisiae",

abstract = "Expression of the glucoamylase-encoding gene (STA2) in Saccharomyces cerevisiae was previously shown to be regulated transcriptionally by both positive and negative factors. The objective of this work was to identify the cis-acting elements responsible for STA2 transcriptional activation as well as the transcriptional repressor effects of STA10 and MATa/MATα. We identified two upstream activation regions (UAS). Three repressor regions responsive to STA10-mediated repression were identified, as well as two regions for down-regulation of STA2 expression. MATa/MATα repression appears to effect STA2 expression either downstream from the translational start site or, indirectly, since no functional al/α2-responsive sequence was identified in the promoter region.",

author = "Lambrechts, {Marius G.} and Pretorius, {Isak S.} and D'Aguanno, {Vincenzo S.} and Paul Sollitti and Julius Marmur",

year = "1994",

month = sep,

day = "2",

doi = "10.1016/0378-1119(94)90286-0",

language = "English",

volume = "146",

pages = "137--144",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Multiple positive and negative cis-acting elements of the STA2 gene regulate glucoamylase synthesis in Saccharomyces cerevisiae

AU - Lambrechts, Marius G.

AU - Pretorius, Isak S.

AU - D'Aguanno, Vincenzo S.

AU - Sollitti, Paul

AU - Marmur, Julius

PY - 1994/9/2

Y1 - 1994/9/2

N2 - Expression of the glucoamylase-encoding gene (STA2) in Saccharomyces cerevisiae was previously shown to be regulated transcriptionally by both positive and negative factors. The objective of this work was to identify the cis-acting elements responsible for STA2 transcriptional activation as well as the transcriptional repressor effects of STA10 and MATa/MATα. We identified two upstream activation regions (UAS). Three repressor regions responsive to STA10-mediated repression were identified, as well as two regions for down-regulation of STA2 expression. MATa/MATα repression appears to effect STA2 expression either downstream from the translational start site or, indirectly, since no functional al/α2-responsive sequence was identified in the promoter region.

AB - Expression of the glucoamylase-encoding gene (STA2) in Saccharomyces cerevisiae was previously shown to be regulated transcriptionally by both positive and negative factors. The objective of this work was to identify the cis-acting elements responsible for STA2 transcriptional activation as well as the transcriptional repressor effects of STA10 and MATa/MATα. We identified two upstream activation regions (UAS). Three repressor regions responsive to STA10-mediated repression were identified, as well as two regions for down-regulation of STA2 expression. MATa/MATα repression appears to effect STA2 expression either downstream from the translational start site or, indirectly, since no functional al/α2-responsive sequence was identified in the promoter region.

UR - http://www.scopus.com/inward/record.url?scp=0027968940&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(94)90286-0

DO - 10.1016/0378-1119(94)90286-0

M3 - Article

C2 - 8076812

AN - SCOPUS:0027968940

SN - 0378-1119

VL - 146

SP - 137

EP - 144

JO - Gene

JF - Gene

IS - 2

ER -

Multiple positive and negative cis-acting elements of the STA2 gene regulate glucoamylase synthesis in Saccharomyces cerevisiae

Abstract

Access to Document

Other files and links

Fingerprint

Cite this