Multiplex detection of ctDNA mutations in plasma of colorectal cancer patients by PCR/SERS assay

Nana Lyu, Vinoth Kumar Rajendran, Russell J. Diefenbach, Kellie Charles, Stephen J. Clarke, Alexander Engel, Sydney 1000 Colorectal Cancer Study Investigators, Helen Rizos, Mark Molloy, Yuling Wang

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Abstract

Molecular diagnostic testing of KRAS and BRAF mutations has become critical in the management of colorectal cancer (CRC) patients. Some progress has been made in liquid biopsy detection of mutations in circulating tumor DNA (ctDNA), which is a fraction of circulating cell-free DNA (cfDNA), but slow analysis for DNA sequencing methods has limited rapid diagnostics. Other methods such as quantitative PCR and more recently, droplet digital PCR (ddPCR), have limitations in multiplexed capacity and the need for expensive specialized equipment. Hence, a robust, rapid and facile strategy is needed for detecting multiple ctDNA mutations to improve the management of CRC patients. To address this significant problem, herein, we propose a new application of multiplex PCR/SERS (surface-enhanced Raman scattering) assay for the detection of ctDNA in CRC, in a fast and non-invasive manner to diagnose and stratify patients for effective treatment.
Original languageEnglish
Pages (from-to)224-232
Number of pages9
JournalNanotheranostics
Volume4
Issue number4
DOIs
Publication statusPublished - 25 Aug 2020

Bibliographical note

Copyright the Author(s) 2020. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • ctDNA
  • CRC
  • PCR/SERS assay
  • ddPCR
  • multiplex detection
  • CtDNA
  • DdPCR
  • Multiplex detection

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