Multiplex detection of ctDNA mutations in plasma of colorectal cancer patients by PCR/SERS assay

Nana Lyu; Vinoth Kumar Rajendran; Russell J. Diefenbach; Kellie Charles; Stephen J. Clarke; Alexander Engel; Sydney 1000 Colorectal Cancer Study Investigators; Helen Rizos; Mark Molloy; Yuling Wang

doi:10.7150/ntno.48905

Multiplex detection of ctDNA mutations in plasma of colorectal cancer patients by PCR/SERS assay

Nana Lyu, Vinoth Kumar Rajendran, Russell J. Diefenbach, Kellie Charles, Stephen J. Clarke, Alexander Engel, Sydney 1000 Colorectal Cancer Study Investigators, Helen Rizos, Mark Molloy, Yuling Wang

Faculty of Science and Engineering

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

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Abstract

Molecular diagnostic testing of KRAS and BRAF mutations has become critical in the management of colorectal cancer (CRC) patients. Some progress has been made in liquid biopsy detection of mutations in circulating tumor DNA (ctDNA), which is a fraction of circulating cell-free DNA (cfDNA), but slow analysis for DNA sequencing methods has limited rapid diagnostics. Other methods such as quantitative PCR and more recently, droplet digital PCR (ddPCR), have limitations in multiplexed capacity and the need for expensive specialized equipment. Hence, a robust, rapid and facile strategy is needed for detecting multiple ctDNA mutations to improve the management of CRC patients. To address this significant problem, herein, we propose a new application of multiplex PCR/SERS (surface-enhanced Raman scattering) assay for the detection of ctDNA in CRC, in a fast and non-invasive manner to diagnose and stratify patients for effective treatment.

Original language	English
Pages (from-to)	224-232
Number of pages	9
Journal	Nanotheranostics
Volume	4
Issue number	4
DOIs	https://doi.org/10.7150/ntno.48905
Publication status	Published - 25 Aug 2020

Bibliographical note

Copyright the Author(s) 2020. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

ctDNA
CRC
PCR/SERS assay
ddPCR
multiplex detection
CtDNA
DdPCR
Multiplex detection

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10.7150/ntno.48905

Publisher version (open access)Final published version, 0.98 MBLicence: CC BY

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Lyu, Nana ; Rajendran, Vinoth Kumar ; Diefenbach, Russell J. ; Charles, Kellie ; Clarke, Stephen J. ; Engel, Alexander ; Sydney 1000 Colorectal Cancer Study Investigators ; Rizos, Helen ; Molloy, Mark ; Wang, Yuling. / Multiplex detection of ctDNA mutations in plasma of colorectal cancer patients by PCR/SERS assay. In: Nanotheranostics. 2020 ; Vol. 4, No. 4. pp. 224-232.

@article{63ebd55ae8134a7893b831a7f0021b5a,

title = "Multiplex detection of ctDNA mutations in plasma of colorectal cancer patients by PCR/SERS assay",

abstract = "Molecular diagnostic testing of KRAS and BRAF mutations has become critical in the management of colorectal cancer (CRC) patients. Some progress has been made in liquid biopsy detection of mutations in circulating tumor DNA (ctDNA), which is a fraction of circulating cell-free DNA (cfDNA), but slow analysis for DNA sequencing methods has limited rapid diagnostics. Other methods such as quantitative PCR and more recently, droplet digital PCR (ddPCR), have limitations in multiplexed capacity and the need for expensive specialized equipment. Hence, a robust, rapid and facile strategy is needed for detecting multiple ctDNA mutations to improve the management of CRC patients. To address this significant problem, herein, we propose a new application of multiplex PCR/SERS (surface-enhanced Raman scattering) assay for the detection of ctDNA in CRC, in a fast and non-invasive manner to diagnose and stratify patients for effective treatment.",

keywords = "ctDNA, CRC, PCR/SERS assay, ddPCR, multiplex detection, CtDNA, DdPCR, Multiplex detection",

author = "Nana Lyu and Rajendran, {Vinoth Kumar} and Diefenbach, {Russell J.} and Kellie Charles and Clarke, {Stephen J.} and Alexander Engel and {Sydney 1000 Colorectal Cancer Study Investigators} and Helen Rizos and Mark Molloy and Yuling Wang and Anthony Gill and Andrew Kneebone and George Hruby and Nick Pavlakis and Connie Diakos and Justin Evans and Shahrir Kabir and Yasser Salama and {Fazekas de St Groth}, Barbara and Margaret Allman-Farinelli and Adrian Bauman and Adrian Smith and Derek Hart and Haryana Dhillon and Madeleine King and Byrne, {Scott N.} and Claudia Rutherford and Andrew Holmes and Nick King and Fatemeh Vafaeem and Helen McGuire",

note = "Copyright the Author(s) 2020. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher. ",

year = "2020",

month = aug,

day = "25",

doi = "10.7150/ntno.48905",

language = "English",

volume = "4",

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Multiplex detection of ctDNA mutations in plasma of colorectal cancer patients by PCR/SERS assay. / Lyu, Nana; Rajendran, Vinoth Kumar; Diefenbach, Russell J.; Charles, Kellie; Clarke, Stephen J.; Engel, Alexander; Sydney 1000 Colorectal Cancer Study Investigators ; Rizos, Helen ; Molloy, Mark ; Wang, Yuling.

In: Nanotheranostics, Vol. 4, No. 4, 25.08.2020, p. 224-232.

Research output: Contribution to journal › Article › peer-review

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AU - Lyu, Nana

AU - Rajendran, Vinoth Kumar

AU - Diefenbach, Russell J.

AU - Charles, Kellie

AU - Clarke, Stephen J.

AU - Engel, Alexander

AU - Sydney 1000 Colorectal Cancer Study Investigators

AU - Rizos, Helen

AU - Molloy, Mark

AU - Wang, Yuling

AU - Gill, Anthony

AU - Kneebone, Andrew

AU - Hruby, George

AU - Pavlakis, Nick

AU - Diakos, Connie

AU - Evans, Justin

AU - Kabir, Shahrir

AU - Salama, Yasser

AU - Fazekas de St Groth, Barbara

AU - Allman-Farinelli, Margaret

AU - Bauman, Adrian

AU - Smith, Adrian

AU - Hart, Derek

AU - Dhillon, Haryana

AU - King, Madeleine

AU - Byrne, Scott N.

AU - Rutherford, Claudia

AU - Holmes, Andrew

AU - King, Nick

AU - Vafaeem, Fatemeh

AU - McGuire, Helen

N1 - Copyright the Author(s) 2020. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2020/8/25

Y1 - 2020/8/25

N2 - Molecular diagnostic testing of KRAS and BRAF mutations has become critical in the management of colorectal cancer (CRC) patients. Some progress has been made in liquid biopsy detection of mutations in circulating tumor DNA (ctDNA), which is a fraction of circulating cell-free DNA (cfDNA), but slow analysis for DNA sequencing methods has limited rapid diagnostics. Other methods such as quantitative PCR and more recently, droplet digital PCR (ddPCR), have limitations in multiplexed capacity and the need for expensive specialized equipment. Hence, a robust, rapid and facile strategy is needed for detecting multiple ctDNA mutations to improve the management of CRC patients. To address this significant problem, herein, we propose a new application of multiplex PCR/SERS (surface-enhanced Raman scattering) assay for the detection of ctDNA in CRC, in a fast and non-invasive manner to diagnose and stratify patients for effective treatment.

AB - Molecular diagnostic testing of KRAS and BRAF mutations has become critical in the management of colorectal cancer (CRC) patients. Some progress has been made in liquid biopsy detection of mutations in circulating tumor DNA (ctDNA), which is a fraction of circulating cell-free DNA (cfDNA), but slow analysis for DNA sequencing methods has limited rapid diagnostics. Other methods such as quantitative PCR and more recently, droplet digital PCR (ddPCR), have limitations in multiplexed capacity and the need for expensive specialized equipment. Hence, a robust, rapid and facile strategy is needed for detecting multiple ctDNA mutations to improve the management of CRC patients. To address this significant problem, herein, we propose a new application of multiplex PCR/SERS (surface-enhanced Raman scattering) assay for the detection of ctDNA in CRC, in a fast and non-invasive manner to diagnose and stratify patients for effective treatment.

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KW - CRC

KW - PCR/SERS assay

KW - ddPCR

KW - multiplex detection

KW - CtDNA

KW - DdPCR

KW - Multiplex detection

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U2 - 10.7150/ntno.48905

DO - 10.7150/ntno.48905

M3 - Article

C2 - 32923312

VL - 4

SP - 224

EP - 232

JO - Nanotheranostics

JF - Nanotheranostics

SN - 2206-7418

IS - 4

ER -

Multiplex detection of ctDNA mutations in plasma of colorectal cancer patients by PCR/SERS assay

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