Nano-ruby

A promising fluorescent probe for background-free cellular imaging

Andrew M. Edmonds, Mushtaq A. Sobhan, Varun K A Sreenivasan, Ekaterina A. Grebenik, James R. Rabeau, Ewa M. Goldys, Andrei V. Zvyagin*

*Corresponding author for this work

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Bioprobes based on fluorescent ruby nanoparticles, which are suitable for ultrasensitive imaging, are reported. A stable aqueous/buffer colloid, permitting facile conjugation to proteins, is produced by femtosecond laser ablation of ruby and the nanoparticles (mean size 17 nm) are photostable, with long lifetime (1-4 ms) 694 nm emission. With time-gating complete (>20 dB) suppression of cell autofluorescence and suppression of exogenous fluorophores is observed. Nanoparticles are imaged in as-grown cells and those immunolabeled with quantum dots. Immunoassay binding to target biomolecules is also demonstrated.

Original languageEnglish
Pages (from-to)506-513
Number of pages8
JournalParticle and Particle Systems Characterization
Volume30
Issue number6
DOIs
Publication statusPublished - Jun 2013

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