Nanochannel gradient separations

Michael A. Startsev, David W. Inglis*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Citation (Scopus)

Abstract

Gradient-based electrophoretic separations enable simultaneous separation and concentration of molecules. Compared with conventional injection-based separations, they enable enrichment of low-concentration analytes from larger sample volumes that are not limited by an injection volume. We have demonstrated that a nanochannel, connecting two chemically different reservoirs, can maintain a stationary chemical gradient while trapping biomolecules and effectively averaging out many of the complex physicochemical hydrodynamics that would broaden the bands in a meso- or microscale capillary. Here we describe chemical and physical methods that enable this work.

Original languageEnglish
Title of host publicationMicrofluidic electrophoresis
Subtitle of host publicationmethods and protocols
EditorsDebashis Dutta
Place of PublicationNew York
PublisherHumana Press
Chapter9
Pages125-132
Number of pages8
ISBN (Electronic)9781493989645
ISBN (Print)9781493989638
DOIs
Publication statusPublished - 2019

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume1906
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Nanofluidic
  • Fabrication
  • Conductivity gradient
  • pH gradient

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