Nanoplasmonic platforms for bioassays

Zygmunt Gryczynski; Evgenia Matveeva; Tanya Shtoyko; Ewa Goldys; John Schetz; Julian Borejdo; Nils Calander; Ignacy Gryczynski

doi:10.1109/METAMAT.2006.335048

Nanoplasmonic platforms for bioassays

Zygmunt Gryczynski^*, Evgenia Matveeva, Tanya Shtoyko, Ewa Goldys, John Schetz, Julian Borejdo, Nils Calander, Ignacy Gryczynski

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

Abstract

Surface-assembled thin molecular layers and nanostructures offer unique opportunities to study processes and mechanisms of macromolecular (protein-protein or/and protein-ligand) interactions. Possibility to capture the conformational information buried in quantum-level interactions offers unique opportunities for the dynamic monitoring of biophysical and biochemical processes at the molecular level. In this report we are presenting novel developments for bioassays with enhanced sensitivity and capabilities due to quantum-photonic interactions of a fluorescent reporter with metallic nanostructures. We discus three cases of plasmons: (1) in nanometer size particles (localized plasmons and metal enhanced fluorescence); (2) in thin metallic film (surface plasmons and directional surface plasmon coupled emeission SPCE); and (3) combination of nanoparticles with continous nanosurfaces (enhanced and directional emission). We will present examples of enhanced immunoassays, background suppression, and applications of this novel technology to the simultaneous measurement of changes in conformation, orientation and mass that occur when biomolecular complexes form.

Original language	English
Title of host publication	2006 International Symposium on Biophotonics, Nanophotonics and Metamaterials
Place of Publication	Piscataway, N.J.
Publisher	Institute of Electrical and Electronics Engineers (IEEE)
Pages	1
Number of pages	1
ISBN (Electronic)	0780397746
ISBN (Print)	0780397738, 9780780397743
DOIs	https://doi.org/10.1109/METAMAT.2006.335048
Publication status	Published - Oct 2006
Event	International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006 - Hangzhou, China Duration: 16 Oct 2006 → 18 Oct 2006

Other

Other	International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006
Country/Territory	China
City	Hangzhou
Period	16/10/06 → 18/10/06

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10.1109/METAMAT.2006.335048

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title = "Nanoplasmonic platforms for bioassays",

abstract = "Surface-assembled thin molecular layers and nanostructures offer unique opportunities to study processes and mechanisms of macromolecular (protein-protein or/and protein-ligand) interactions. Possibility to capture the conformational information buried in quantum-level interactions offers unique opportunities for the dynamic monitoring of biophysical and biochemical processes at the molecular level. In this report we are presenting novel developments for bioassays with enhanced sensitivity and capabilities due to quantum-photonic interactions of a fluorescent reporter with metallic nanostructures. We discus three cases of plasmons: (1) in nanometer size particles (localized plasmons and metal enhanced fluorescence); (2) in thin metallic film (surface plasmons and directional surface plasmon coupled emeission SPCE); and (3) combination of nanoparticles with continous nanosurfaces (enhanced and directional emission). We will present examples of enhanced immunoassays, background suppression, and applications of this novel technology to the simultaneous measurement of changes in conformation, orientation and mass that occur when biomolecular complexes form.",

author = "Zygmunt Gryczynski and Evgenia Matveeva and Tanya Shtoyko and Ewa Goldys and John Schetz and Julian Borejdo and Nils Calander and Ignacy Gryczynski",

year = "2006",

month = oct,

doi = "10.1109/METAMAT.2006.335048",

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booktitle = "2006 International Symposium on Biophotonics, Nanophotonics and Metamaterials",

publisher = "Institute of Electrical and Electronics Engineers (IEEE)",

address = "United States",

note = "International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006 ; Conference date: 16-10-2006 Through 18-10-2006",

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Gryczynski, Z, Matveeva, E, Shtoyko, T, Goldys, E, Schetz, J, Borejdo, J, Calander, N & Gryczynski, I 2006, Nanoplasmonic platforms for bioassays. in 2006 International Symposium on Biophotonics, Nanophotonics and Metamaterials., 4134787, Institute of Electrical and Electronics Engineers (IEEE), Piscataway, N.J., pp. 1, International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006, Hangzhou, China, 16/10/06. https://doi.org/10.1109/METAMAT.2006.335048

Nanoplasmonic platforms for bioassays. / Gryczynski, Zygmunt; Matveeva, Evgenia; Shtoyko, Tanya et al.
2006 International Symposium on Biophotonics, Nanophotonics and Metamaterials. Piscataway, N.J.: Institute of Electrical and Electronics Engineers (IEEE), 2006. p. 1 4134787.

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

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AU - Gryczynski, Zygmunt

AU - Matveeva, Evgenia

AU - Shtoyko, Tanya

AU - Goldys, Ewa

AU - Schetz, John

AU - Borejdo, Julian

AU - Calander, Nils

AU - Gryczynski, Ignacy

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N2 - Surface-assembled thin molecular layers and nanostructures offer unique opportunities to study processes and mechanisms of macromolecular (protein-protein or/and protein-ligand) interactions. Possibility to capture the conformational information buried in quantum-level interactions offers unique opportunities for the dynamic monitoring of biophysical and biochemical processes at the molecular level. In this report we are presenting novel developments for bioassays with enhanced sensitivity and capabilities due to quantum-photonic interactions of a fluorescent reporter with metallic nanostructures. We discus three cases of plasmons: (1) in nanometer size particles (localized plasmons and metal enhanced fluorescence); (2) in thin metallic film (surface plasmons and directional surface plasmon coupled emeission SPCE); and (3) combination of nanoparticles with continous nanosurfaces (enhanced and directional emission). We will present examples of enhanced immunoassays, background suppression, and applications of this novel technology to the simultaneous measurement of changes in conformation, orientation and mass that occur when biomolecular complexes form.

AB - Surface-assembled thin molecular layers and nanostructures offer unique opportunities to study processes and mechanisms of macromolecular (protein-protein or/and protein-ligand) interactions. Possibility to capture the conformational information buried in quantum-level interactions offers unique opportunities for the dynamic monitoring of biophysical and biochemical processes at the molecular level. In this report we are presenting novel developments for bioassays with enhanced sensitivity and capabilities due to quantum-photonic interactions of a fluorescent reporter with metallic nanostructures. We discus three cases of plasmons: (1) in nanometer size particles (localized plasmons and metal enhanced fluorescence); (2) in thin metallic film (surface plasmons and directional surface plasmon coupled emeission SPCE); and (3) combination of nanoparticles with continous nanosurfaces (enhanced and directional emission). We will present examples of enhanced immunoassays, background suppression, and applications of this novel technology to the simultaneous measurement of changes in conformation, orientation and mass that occur when biomolecular complexes form.

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BT - 2006 International Symposium on Biophotonics, Nanophotonics and Metamaterials

PB - Institute of Electrical and Electronics Engineers (IEEE)

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T2 - International Symposium on Biophotonics, Nanophotonics and Metamaterials, Metamaterials 2006

Y2 - 16 October 2006 through 18 October 2006

ER -

Nanoplasmonic platforms for bioassays

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