Neuronal cell lines as model dorsal root ganglion neurons: a transcriptomic comparison

Kathleen Yin, Gregory J. Baillie, Irina Vetter

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Background: Dorsal root ganglion neuron-derived immortal cell lines including ND7/23 and F-11 cells have been used extensively as in vitro model systems of native peripheral sensory neurons. However, while it is clear that some sensory neuron-specific receptors and ion channels are present in these cell lines, a systematic comparison of the molecular targets expressed by these cell lines with those expressed in intact peripheral neurons is lacking. 

Results: In this study, we examined the expression of RNA transcripts in the human neuroblastoma-derived cell line, SH-SY5Y, and two dorsal root ganglion hybridoma cell lines, F-11 and ND7/23, using Illumina next-generation sequencing, and compared the results with native whole murine dorsal root ganglions. The gene expression profiles of these three cell lines did not resemble any specific defined dorsal root ganglion subclass. The cell lines lacked many markers for nociceptive sensory neurons, such as the Transient receptor potential V1 gene, but expressed markers for both myelinated and unmyelinated neurons. Global gene ontology analysis on whole dorsal root ganglions and cell lines showed similar enrichment of biological process terms across all samples. 

Conclusions: This paper provides insights into the receptor repertoire expressed in common dorsal root ganglion neuron-derived cell lines compared with whole murine dorsal root ganglions, and illustrates the limits and potentials of these cell lines as tools for neuropharmacological exploration.

LanguageEnglish
Pages1-17
Number of pages17
JournalMolecular Pain
Volume12
DOIs
Publication statusPublished - 29 Apr 2016
Externally publishedYes

Fingerprint

Spinal Ganglia
Neurons
Cell Line
Sensory Receptor Cells
Biological Phenomena
Vasopressin Receptors
Nociceptors
Gene Ontology
Hybridomas
Neuroblastoma
Ion Channels
Transcriptome
RNA

Bibliographical note

Copyright the Author(s) 2016. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • dorsal root ganglions
  • SH-SY5Y
  • F-11
  • ND7/23
  • transcriptomic analysis
  • molecular expression

Cite this

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abstract = "Background: Dorsal root ganglion neuron-derived immortal cell lines including ND7/23 and F-11 cells have been used extensively as in vitro model systems of native peripheral sensory neurons. However, while it is clear that some sensory neuron-specific receptors and ion channels are present in these cell lines, a systematic comparison of the molecular targets expressed by these cell lines with those expressed in intact peripheral neurons is lacking. Results: In this study, we examined the expression of RNA transcripts in the human neuroblastoma-derived cell line, SH-SY5Y, and two dorsal root ganglion hybridoma cell lines, F-11 and ND7/23, using Illumina next-generation sequencing, and compared the results with native whole murine dorsal root ganglions. The gene expression profiles of these three cell lines did not resemble any specific defined dorsal root ganglion subclass. The cell lines lacked many markers for nociceptive sensory neurons, such as the Transient receptor potential V1 gene, but expressed markers for both myelinated and unmyelinated neurons. Global gene ontology analysis on whole dorsal root ganglions and cell lines showed similar enrichment of biological process terms across all samples. Conclusions: This paper provides insights into the receptor repertoire expressed in common dorsal root ganglion neuron-derived cell lines compared with whole murine dorsal root ganglions, and illustrates the limits and potentials of these cell lines as tools for neuropharmacological exploration.",
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Neuronal cell lines as model dorsal root ganglion neurons : a transcriptomic comparison. / Yin, Kathleen; Baillie, Gregory J.; Vetter, Irina.

In: Molecular Pain, Vol. 12, 29.04.2016, p. 1-17.

Research output: Contribution to journalArticleResearchpeer-review

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