Neuropeptides encoded by the genomes of the akoya pearl oyster pinctata fucata and pacific oyster crassostrea gigas

a bioinformatic and peptidomic survey

Michael J. Stewart, Pascal Favrel, Bronwyn A. Rotgans, Tianfang Wang, Min Zhao, Manzar Sohail, Wayne A. O'Connor, Abigail Elizur, Joel Henry, Scott F. Cummins*

*Corresponding author for this work

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Background: Oysters impart significant socio-ecological benefits from primary production of food supply, to estuarine ecosystems via reduction of water column nutrients, plankton and seston biomass. Little though is known at the molecular level of what genes are responsible for how oysters reproduce, filter nutrients, survive stressful physiological events and form reef communities. Neuropeptides represent a diverse class of chemical messengers, instrumental in orchestrating these complex physiological events in other species. Results: By a combination of in silico data mining and peptide analysis of ganglia, 74 putative neuropeptide genes were identified from genome and transcriptome databases of the Akoya pearl oyster, Pinctata fucata and the Pacific oyster, Crassostrea gigas, encoding precursors for over 300 predicted bioactive peptide products, including three newly identified neuropeptide precursors PFGx8amide, RxIamide and Wx3Yamide. Our findings also include a gene for the gonadotropin-releasing hormone (GnRH) and two egg-laying hormones (ELH) which were identified from both oysters. Multiple sequence alignments and phylogenetic analysis supports similar global organization of these mature peptides. Computer-based peptide modeling of the molecular tertiary structures of ELH highlights the structural homologies within ELH family, which may facilitate ELH activity leading to the release of gametes. Conclusion: Our analysis demonstrates that oysters possess conserved molluscan neuropeptide domains and overall precursor organization whilst highlighting many previously unrecognized bivalve idiosyncrasies. This genomic analysis provides a solid foundation from which further studies aimed at the functional characterization of these molluscan neuropeptides can be conducted to further stimulate advances in understanding the ecology and cultivation of oysters.

Original languageEnglish
Article number840
Pages (from-to)1-16
Number of pages16
JournalBMC Genetics
Volume15
DOIs
Publication statusPublished - 2 Oct 2014
Externally publishedYes

Bibliographical note

Copyright the Author(s) 2014. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • Circular dichroism
  • Crassostrea gigas
  • Egg-laying hormone
  • Feed circuit activating peptide
  • Gonadotropin-releasing hormone
  • High-performance liquid chromatography
  • Mass spectrometry
  • Molluscs
  • Neuropeptides
  • Pinctada fucata

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