Neuroprotective effects of rosmarinic acid on ciguatoxin in primary human neurons

N. Braidy, A. Matin, F. Rossi, M. Chinain, D. Laurent, G. J. Guillemin*

*Corresponding author for this work

    Research output: Contribution to journalArticle

    26 Citations (Scopus)

    Abstract

    Ciguatoxin (CTX), is a toxic compound produced by microalgae (dinoflagellate) Gambierdiscus spp., and is bio-accumulated and bio-transformed through the marine food chain causing neurological deficits. To determine the mechanism of CTX-mediated cytotoxicity in human neurons, we measured extracellular lactate dehydrogenase (LDH) activity, intracellular levels of nicotinamide adenine dinucleotide (NAD+) and H2AX phosphorylation at serine 139 as a measure for DNA damage in primary cultures of human neurons treated with Pacific (P)-CTX-1B and P-CTX-3C. We found these marine toxins can induce a time and dose-dependent increase in extracellular LDH activity, with a concomitant decline in intracellular NAD+ levels and increased DNA damage at the concentration range of 5-200 nM. We also showed that pre- and post-treatment with rosmarinic acid (RA), the active constituent of the Heliotropium foertherianum (Boraginaceae) can attenuate CTX-mediated neurotoxicity. These results further highlight the potential of RA in the treatment of CTX-induced neurological deficits.

    Original languageEnglish
    Pages (from-to)226-234
    Number of pages9
    JournalNeurotoxicity Research
    Volume25
    Issue number2
    DOIs
    Publication statusPublished - Feb 2014

    Fingerprint Dive into the research topics of 'Neuroprotective effects of rosmarinic acid on ciguatoxin in primary human neurons'. Together they form a unique fingerprint.

  • Cite this