New insights into the phenotype of human dendritic cell populations

Georgina Jane Clark, Fiona Kupresanin, Phillip Dieter Fromm, Xinsheng Ju, Leticia Muusers, Pablo Alejandro Silveira, Zehra Elgundi, Robin Edward Gasiorowski, Michael Stefan Papadimitrious, Christian Bryant, Kenneth Maan Liong Lee, Candice Jane Clarke, James William Young, Andrew Chan, Andrew Harman, Rachel Botting, Raquel Cabezon, Daniel Benitez-Ribas, Anna Elizabeth Stella Brooks, Phillip Rodney DunbarDerek Nigel John Hart

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)
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Abstract

HLDA10 is the Tenth Human Leukocyte Differentiation Antigen (HLDA) Workshop. The HLDA Workshops provide a mechanism to allocate cluster of differentiation (CD) nomenclature by engaging in interlaboratory studies. As the host laboratory, we invited researchers from national and international academic and commercial institutions to submit monoclonal antibodies (mAbs) to human leukocyte surface membrane molecules, particularly those that recognised molecules on human myeloid cell populations and dendritic cells (DCs). These mAbs were tested for activity and then distributed as a blinded panel to 15 international laboratories to test on different leukocyte populations. These populations included blood DCs, skin-derived DCs, tonsil leukocytes, monocyte-derived DCs, CD34-derived DCs, macrophage populations and diagnostic acute myeloid leukaemia and lymphoma samples. Each laboratory was provided with enough mAb to perform five repeat experiments. Here, we summarise the reactivity of different mAb to 68 different cell-surface molecules expressed by human myeloid and DC populations. Submitted mAbs to some of the molecules were further validated to collate data required to designate a formal CD number. This collaborative process provides the broader scientific community with an invaluable data set validating mAbs to leukocyte-surface molecules.
Original languageEnglish
Article numbere61
Pages (from-to)1-12
Number of pages12
JournalClinical and Translational Immunology
Volume5
Issue number1
DOIs
Publication statusPublished - 1 Jan 2016
Externally publishedYes

Bibliographical note

Copyright 2016 Australasian Society for Immunology Inc. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

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