TY - JOUR
T1 - New insights into the phenotype of human dendritic cell populations
AU - Clark, Georgina Jane
AU - Kupresanin, Fiona
AU - Fromm, Phillip Dieter
AU - Ju, Xinsheng
AU - Muusers, Leticia
AU - Silveira, Pablo Alejandro
AU - Elgundi, Zehra
AU - Gasiorowski, Robin Edward
AU - Papadimitrious, Michael Stefan
AU - Bryant, Christian
AU - Lee, Kenneth Maan Liong
AU - Clarke, Candice Jane
AU - Young, James William
AU - Chan, Andrew
AU - Harman, Andrew
AU - Botting, Rachel
AU - Cabezon, Raquel
AU - Benitez-Ribas, Daniel
AU - Brooks, Anna Elizabeth Stella
AU - Dunbar, Phillip Rodney
AU - Hart, Derek Nigel John
N1 - Copyright 2016 Australasian Society for Immunology Inc. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.
PY - 2016/1/1
Y1 - 2016/1/1
N2 - HLDA10 is the Tenth Human Leukocyte Differentiation Antigen (HLDA) Workshop. The HLDA Workshops provide a mechanism to allocate cluster of differentiation (CD) nomenclature by engaging in interlaboratory studies. As the host laboratory, we invited researchers from national and international academic and commercial institutions to submit monoclonal antibodies (mAbs) to human leukocyte surface membrane molecules, particularly those that recognised molecules on human myeloid cell populations and dendritic cells (DCs). These mAbs were tested for activity and then distributed as a blinded panel to 15 international laboratories to test on different leukocyte populations. These populations included blood DCs, skin-derived DCs, tonsil leukocytes, monocyte-derived DCs, CD34-derived DCs, macrophage populations and diagnostic acute myeloid leukaemia and lymphoma samples. Each laboratory was provided with enough mAb to perform five repeat experiments. Here, we summarise the reactivity of different mAb to 68 different cell-surface molecules expressed by human myeloid and DC populations. Submitted mAbs to some of the molecules were further validated to collate data required to designate a formal CD number. This collaborative process provides the broader scientific community with an invaluable data set validating mAbs to leukocyte-surface molecules.
AB - HLDA10 is the Tenth Human Leukocyte Differentiation Antigen (HLDA) Workshop. The HLDA Workshops provide a mechanism to allocate cluster of differentiation (CD) nomenclature by engaging in interlaboratory studies. As the host laboratory, we invited researchers from national and international academic and commercial institutions to submit monoclonal antibodies (mAbs) to human leukocyte surface membrane molecules, particularly those that recognised molecules on human myeloid cell populations and dendritic cells (DCs). These mAbs were tested for activity and then distributed as a blinded panel to 15 international laboratories to test on different leukocyte populations. These populations included blood DCs, skin-derived DCs, tonsil leukocytes, monocyte-derived DCs, CD34-derived DCs, macrophage populations and diagnostic acute myeloid leukaemia and lymphoma samples. Each laboratory was provided with enough mAb to perform five repeat experiments. Here, we summarise the reactivity of different mAb to 68 different cell-surface molecules expressed by human myeloid and DC populations. Submitted mAbs to some of the molecules were further validated to collate data required to designate a formal CD number. This collaborative process provides the broader scientific community with an invaluable data set validating mAbs to leukocyte-surface molecules.
UR - http://www.scopus.com/inward/record.url?scp=84981544046&partnerID=8YFLogxK
U2 - 10.1038/cti.2015.40
DO - 10.1038/cti.2015.40
M3 - Article
C2 - 26900474
SN - 2050-0068
VL - 5
SP - 1
EP - 12
JO - Clinical and Translational Immunology
JF - Clinical and Translational Immunology
IS - 1
M1 - e61
ER -