New mobile gene cassettes containing an aminoglycoside resistance gene, aacA7, and a chloramphenicol resistance gene, catB3, in an integron in pBWH301

K. L. Bunny, R. M. Hall, H. W. Stokes*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    100 Citations (Scopus)

    Abstract

    The multidrug resistance plasmid pBWH301 was shown to contain a sulI- associated integron with five inserted gene cassettes, aacA7-catB3-aadB- oxa2-orfD, all of which can be mobilized by the integron-encoded DNA integrase. The aadB, oxa2, and orfD cassettes are identical to known cassettes. The aacA7 gene encodes a protein that is a member of one of the three known families of aminoglycoside acetyltransferases classified as AAC(6')-I. The chloramphenicol acetyltransferase encoded by the catB3 gene is closely related to members of a recently identified family of chloramphenicol acetyltransferases. The catB3 gene displays a relatively high degree of sequence identity to a chromosomally located open reading frame in Pseudomonas aeruginosa, and this may represent evidence for the acquisition by a cassette of a chromosomal gene.

    Original languageEnglish
    Pages (from-to)686-693
    Number of pages8
    JournalAntimicrobial Agents and Chemotherapy
    Volume39
    Issue number3
    DOIs
    Publication statusPublished - Mar 1995

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