Next generation barcode tagged sequencing for monitoring microbial community dynamics

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Abstract

Microbial identification using 16S rDNA variable regions has become increasingly popular over the past decade. The application of next-generation amplicon sequencing to these regions allows microbial communities to be sequenced in far greater depth than previous techniques, as well as allowing for the identification of unculturable or rare organisms within a sample. Multiplexing can be used to sequence multiple samples in tandem through the use of sample-specific identification sequences which are attached to each amplicon, making this a cost-effective method for large-scale microbial identification experiments.

Original languageEnglish
Title of host publicationEnvironmental Microbiology
Subtitle of host publicationMethods and Protocols
EditorsIan T. Paulsen, Andrew J. Holmes
Place of PublicationTotowa, NJ
PublisherHumana Press
Pages101-110
Number of pages10
Edition2nd
ISBN (Electronic)9781627037129
ISBN (Print)9781627037112
DOIs
Publication statusPublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1096
ISSN (Print)1064-3745

Keywords

  • Amplicon sequencing
  • D454
  • K16S rDNA
  • Microbial identification
  • Multiplex sequencing

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