Next generation barcode tagged sequencing for monitoring microbial community dynamics

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    2 Citations (Scopus)


    Microbial identification using 16S rDNA variable regions has become increasingly popular over the past decade. The application of next-generation amplicon sequencing to these regions allows microbial communities to be sequenced in far greater depth than previous techniques, as well as allowing for the identification of unculturable or rare organisms within a sample. Multiplexing can be used to sequence multiple samples in tandem through the use of sample-specific identification sequences which are attached to each amplicon, making this a cost-effective method for large-scale microbial identification experiments.

    Original languageEnglish
    Title of host publicationEnvironmental Microbiology
    Subtitle of host publicationMethods and Protocols
    EditorsIan T. Paulsen, Andrew J. Holmes
    Place of PublicationTotowa, NJ
    PublisherHumana Press
    Number of pages10
    ISBN (Electronic)9781627037129
    ISBN (Print)9781627037112
    Publication statusPublished - 2014

    Publication series

    NameMethods in Molecular Biology
    ISSN (Print)1064-3745


    • Amplicon sequencing
    • D454
    • K16S rDNA
    • Microbial identification
    • Multiplex sequencing


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