Next generation sequence analysis of the transcriptome of Sydney rock oysters (Saccostrea glomerata) exposed to a range of environmental stressors

Sharon E. Hook; Emma L. Johnston; Sham Nair; Anthony C. Roach; Philippe Moncuquet; Natalie A. Twine; David A. Raftos

doi:10.1016/j.margen.2014.08.003

Next generation sequence analysis of the transcriptome of Sydney rock oysters (Saccostrea glomerata) exposed to a range of environmental stressors

Sharon E. Hook^*, Emma L. Johnston, Sham Nair, Anthony C. Roach, Philippe Moncuquet, Natalie A. Twine, David A. Raftos

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Sydney rock oysters (Saccostrea glomerata) were exposed to environmental stressors at contaminated field sites or in a controlled laboratory setting. RNA seq transcriptome data were generated for the gill and digestive gland using Roche's 454 pyrosequencing technology. 28,685 contigs were de novo assembled which encoded 11,671 different protein products. The data will act as a reference for future studies in ecology, immunology and environmental toxicology.

Original language	English
Pages (from-to)	109-111
Number of pages	3
Journal	Marine Genomics
Volume	18
Issue number	PB
DOIs	https://doi.org/10.1016/j.margen.2014.08.003
Publication status	Published - 1 Dec 2014

Keywords

454 pyrosequencing
Immune response
Metals
Oil
Oyster
RNA seq

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10.1016/j.margen.2014.08.003

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title = "Next generation sequence analysis of the transcriptome of Sydney rock oysters (Saccostrea glomerata) exposed to a range of environmental stressors",

abstract = "Sydney rock oysters (Saccostrea glomerata) were exposed to environmental stressors at contaminated field sites or in a controlled laboratory setting. RNA seq transcriptome data were generated for the gill and digestive gland using Roche's 454 pyrosequencing technology. 28,685 contigs were de novo assembled which encoded 11,671 different protein products. The data will act as a reference for future studies in ecology, immunology and environmental toxicology.",

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AU - Hook, Sharon E.

AU - Johnston, Emma L.

AU - Nair, Sham

AU - Roach, Anthony C.

AU - Moncuquet, Philippe

AU - Twine, Natalie A.

AU - Raftos, David A.

PY - 2014/12/1

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AB - Sydney rock oysters (Saccostrea glomerata) were exposed to environmental stressors at contaminated field sites or in a controlled laboratory setting. RNA seq transcriptome data were generated for the gill and digestive gland using Roche's 454 pyrosequencing technology. 28,685 contigs were de novo assembled which encoded 11,671 different protein products. The data will act as a reference for future studies in ecology, immunology and environmental toxicology.

KW - 454 pyrosequencing

KW - Immune response

KW - Metals

KW - Oil

KW - Oyster

KW - RNA seq

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SN - 1874-7787

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JO - Marine Genomics

JF - Marine Genomics

IS - PB

ER -

Next generation sequence analysis of the transcriptome of Sydney rock oysters (Saccostrea glomerata) exposed to a range of environmental stressors

Abstract

Keywords

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