Nigrostriatal dopamine release modulated by mesopontine muscarinic receptors

Anthony D. Miller, Charles D. Blaha*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

The present study investigated the regulation of substantia nigra pars compacta (SNc) dopamine neuronal activity by pedunculopontine (PPT) cholinergic neurons. Changes in dopamine efflux following chemical activation or blockade of muscarinic acetylcholine receptors in the PPT were measured at stearate-carbon paste electrodes in the striatum of urethane (1.5 g/kg) anaesthetized male rats using in vivo chronoamperometry (30s sampling rate). Intra-PPT infusions of a mixed muscarinic/nicotinic (carbachol 8 μg/μl) or M2/4-selective muscarinic (oxotremorine 0.5 μg/μl) receptor agonist attenuated striatal dopamine efflux, whereas a non-selective (scopolamine 100 μg/μl) or M2/4-selective (methoctramine 50 μg/μl) muscarinic receptor antagonist enhanced striatal dopamine efflux. These results suggest that M2/4 muscarinic receptors in the mesopontine tonically influence SNc basal dopamine cell activity and striatal dopamine release.

Original languageEnglish
Pages (from-to)1805-1808
Number of pages4
JournalNeuroReport
Volume15
Issue number11
DOIs
Publication statusPublished - 6 Aug 2004

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