Nitrogen assimilation in the symbiotic marine alga Gymnodinium microadriaticum

direct analysis of 15N incorporation by gc-ms methods

Roger E. Summons*, C. Barry Osmond

*Corresponding author for this work

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    15N-Labelled ammonium chloride, sodium nitrate and urea were supplied in vitro to freshly isolated Gymnodinium microadriaticum, the algal symbiont (zooxanthellae) of the clam Hippopus hippopus. Subsequent extraction ofthe resulting labelled amino acids and determination ofthe distribution of 15N in glutamine, glutamic acid and aspartic acid, using gas chromatography-mass spectrometry revealed a light dependent assimilation from all substrates, primarily via the GS-GOGAT pathway.

    Original languageEnglish
    Pages (from-to)575-578
    Number of pages4
    JournalPhytochemistry
    Volume20
    Issue number4
    DOIs
    Publication statusPublished - 1981

    Keywords

    • gas chromatography-mass spectrometry.
    • Gymnodinium microadriaticum
    • nitrogen assimilation
    • stable isotopes
    • zooxanthellae

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