Nitrogen assimilation in the symbiotic marine alga Gymnodinium microadriaticum: direct analysis of 15N incorporation by gc-ms methods

Roger E. Summons; C. Barry Osmond

doi:10.1016/0031-9422(81)85136-9

Nitrogen assimilation in the symbiotic marine alga Gymnodinium microadriaticum: direct analysis of 15N incorporation by gc-ms methods

Roger E. Summons^*, C. Barry Osmond

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

¹⁵N-Labelled ammonium chloride, sodium nitrate and urea were supplied in vitro to freshly isolated Gymnodinium microadriaticum, the algal symbiont (zooxanthellae) of the clam Hippopus hippopus. Subsequent extraction ofthe resulting labelled amino acids and determination ofthe distribution of ¹⁵N in glutamine, glutamic acid and aspartic acid, using gas chromatography-mass spectrometry revealed a light dependent assimilation from all substrates, primarily via the GS-GOGAT pathway.

Original language	English
Pages (from-to)	575-578
Number of pages	4
Journal	Phytochemistry
Volume	20
Issue number	4
DOIs	https://doi.org/10.1016/0031-9422(81)85136-9
Publication status	Published - 1981

Keywords

gas chromatography-mass spectrometry.
Gymnodinium microadriaticum
nitrogen assimilation
stable isotopes
zooxanthellae

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10.1016/0031-9422(81)85136-9

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title = "Nitrogen assimilation in the symbiotic marine alga Gymnodinium microadriaticum: direct analysis of 15N incorporation by gc-ms methods",

abstract = " 15N-Labelled ammonium chloride, sodium nitrate and urea were supplied in vitro to freshly isolated Gymnodinium microadriaticum, the algal symbiont (zooxanthellae) of the clam Hippopus hippopus. Subsequent extraction ofthe resulting labelled amino acids and determination ofthe distribution of 15N in glutamine, glutamic acid and aspartic acid, using gas chromatography-mass spectrometry revealed a light dependent assimilation from all substrates, primarily via the GS-GOGAT pathway.",

keywords = "gas chromatography-mass spectrometry., Gymnodinium microadriaticum, nitrogen assimilation, stable isotopes, zooxanthellae",

author = "Summons, \{Roger E.\} and Osmond, \{C. Barry\}",

year = "1981",

doi = "10.1016/0031-9422(81)85136-9",

language = "English",

volume = "20",

pages = "575--578",

journal = "Phytochemistry",

issn = "0031-9422",

publisher = "Elsevier",

number = "4",

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TY - JOUR

T1 - Nitrogen assimilation in the symbiotic marine alga Gymnodinium microadriaticum

T2 - direct analysis of 15N incorporation by gc-ms methods

AU - Summons, Roger E.

AU - Osmond, C. Barry

PY - 1981

Y1 - 1981

N2 - 15N-Labelled ammonium chloride, sodium nitrate and urea were supplied in vitro to freshly isolated Gymnodinium microadriaticum, the algal symbiont (zooxanthellae) of the clam Hippopus hippopus. Subsequent extraction ofthe resulting labelled amino acids and determination ofthe distribution of 15N in glutamine, glutamic acid and aspartic acid, using gas chromatography-mass spectrometry revealed a light dependent assimilation from all substrates, primarily via the GS-GOGAT pathway.

AB - 15N-Labelled ammonium chloride, sodium nitrate and urea were supplied in vitro to freshly isolated Gymnodinium microadriaticum, the algal symbiont (zooxanthellae) of the clam Hippopus hippopus. Subsequent extraction ofthe resulting labelled amino acids and determination ofthe distribution of 15N in glutamine, glutamic acid and aspartic acid, using gas chromatography-mass spectrometry revealed a light dependent assimilation from all substrates, primarily via the GS-GOGAT pathway.

KW - gas chromatography-mass spectrometry.

KW - Gymnodinium microadriaticum

KW - nitrogen assimilation

KW - stable isotopes

KW - zooxanthellae

UR - https://www.scopus.com/pages/publications/0001410399

U2 - 10.1016/0031-9422(81)85136-9

DO - 10.1016/0031-9422(81)85136-9

M3 - Article

AN - SCOPUS:0001410399

SN - 0031-9422

VL - 20

SP - 575

EP - 578

JO - Phytochemistry

JF - Phytochemistry

IS - 4

ER -

Nitrogen assimilation in the symbiotic marine alga Gymnodinium microadriaticum: direct analysis of 15N incorporation by gc-ms methods

Abstract

Keywords

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