Non-canonical regulation of phosphatidylinositol 3-kinase gamma isoform activity in retinal rod photoreceptor cells

Vivek K. Gupta, Ammaji Rajala, Raju V S Rajala

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Phosphatidylinositol 3-Kinases (PI3Ks) are a family of lipid kinases that phosphorylate the D3-hydroxyls of the inositol ring of phosphoinositides, and are responsible for coordinating a diverse range of cellular functions. A canonical pathway of activation of PI3Ks through the interaction of RA-domain with Ras proteins has been well established. In retinal photoreceptors, we have identified a non-canonical pathway of PI3Kγ activation through the interaction of its RA-domain with a putative Ras-like domain (RLD) in alpha subunit of cyclic nucleotide-gated channel (CNGA1) in retinal rod photoreceptors.

Results: The interaction between PI3Kγ and CNGA1 does not appear to play a role in regulation of CNG channel activity, but PI3Kγ uses CNGA1 as an anchoring module to achieve close proximity to its substrate to generate D3-phosphoinositides.

Conclusions: Our studies suggest a functional non-canonical PI3Kγ activation in retinal rod photoreceptor cells.

Original languageEnglish
Article number7
Pages (from-to)1-11
Number of pages11
JournalCell Communication and Signaling
Volume13
DOIs
Publication statusPublished - 2015
Externally publishedYes

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