Non-canonical regulation of phosphatidylinositol 3-kinase gamma isoform activity in retinal rod photoreceptor cells

Vivek K. Gupta; Ammaji Rajala; Raju V S Rajala

doi:10.1186/s12964-015-0087-9

Non-canonical regulation of phosphatidylinositol 3-kinase gamma isoform activity in retinal rod photoreceptor cells

Vivek K. Gupta
, Ammaji Rajala
, Raju V S Rajala

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Background: Phosphatidylinositol 3-Kinases (PI3Ks) are a family of lipid kinases that phosphorylate the D3-hydroxyls of the inositol ring of phosphoinositides, and are responsible for coordinating a diverse range of cellular functions. A canonical pathway of activation of PI3Ks through the interaction of RA-domain with Ras proteins has been well established. In retinal photoreceptors, we have identified a non-canonical pathway of PI3Kγ activation through the interaction of its RA-domain with a putative Ras-like domain (RLD) in alpha subunit of cyclic nucleotide-gated channel (CNGA1) in retinal rod photoreceptors.

Results: The interaction between PI3Kγ and CNGA1 does not appear to play a role in regulation of CNG channel activity, but PI3Kγ uses CNGA1 as an anchoring module to achieve close proximity to its substrate to generate D3-phosphoinositides.

Conclusions: Our studies suggest a functional non-canonical PI3Kγ activation in retinal rod photoreceptor cells.

Original language	English
Article number	7
Pages (from-to)	1-11
Number of pages	11
Journal	Cell Communication and Signaling
Volume	13
DOIs	https://doi.org/10.1186/s12964-015-0087-9
Publication status	Published - 2015
Externally published	Yes

Access to Document

10.1186/s12964-015-0087-9

Cite this

@article{8075f21a5b3d4b2f9a918d02470eb1dd,

title = "Non-canonical regulation of phosphatidylinositol 3-kinase gamma isoform activity in retinal rod photoreceptor cells",

abstract = "Background: Phosphatidylinositol 3-Kinases (PI3Ks) are a family of lipid kinases that phosphorylate the D3-hydroxyls of the inositol ring of phosphoinositides, and are responsible for coordinating a diverse range of cellular functions. A canonical pathway of activation of PI3Ks through the interaction of RA-domain with Ras proteins has been well established. In retinal photoreceptors, we have identified a non-canonical pathway of PI3Kγ activation through the interaction of its RA-domain with a putative Ras-like domain (RLD) in alpha subunit of cyclic nucleotide-gated channel (CNGA1) in retinal rod photoreceptors.Results: The interaction between PI3Kγ and CNGA1 does not appear to play a role in regulation of CNG channel activity, but PI3Kγ uses CNGA1 as an anchoring module to achieve close proximity to its substrate to generate D3-phosphoinositides.Conclusions: Our studies suggest a functional non-canonical PI3Kγ activation in retinal rod photoreceptor cells.",

author = "Gupta, \{Vivek K.\} and Ammaji Rajala and Rajala, \{Raju V S\}",

year = "2015",

doi = "10.1186/s12964-015-0087-9",

language = "English",

volume = "13",

pages = "1--11",

journal = "Cell Communication and Signaling",

issn = "1478-811X",

publisher = "Springer, Springer Nature",

}

TY - JOUR

T1 - Non-canonical regulation of phosphatidylinositol 3-kinase gamma isoform activity in retinal rod photoreceptor cells

AU - Gupta, Vivek K.

AU - Rajala, Ammaji

AU - Rajala, Raju V S

PY - 2015

Y1 - 2015

N2 - Background: Phosphatidylinositol 3-Kinases (PI3Ks) are a family of lipid kinases that phosphorylate the D3-hydroxyls of the inositol ring of phosphoinositides, and are responsible for coordinating a diverse range of cellular functions. A canonical pathway of activation of PI3Ks through the interaction of RA-domain with Ras proteins has been well established. In retinal photoreceptors, we have identified a non-canonical pathway of PI3Kγ activation through the interaction of its RA-domain with a putative Ras-like domain (RLD) in alpha subunit of cyclic nucleotide-gated channel (CNGA1) in retinal rod photoreceptors.Results: The interaction between PI3Kγ and CNGA1 does not appear to play a role in regulation of CNG channel activity, but PI3Kγ uses CNGA1 as an anchoring module to achieve close proximity to its substrate to generate D3-phosphoinositides.Conclusions: Our studies suggest a functional non-canonical PI3Kγ activation in retinal rod photoreceptor cells.

AB - Background: Phosphatidylinositol 3-Kinases (PI3Ks) are a family of lipid kinases that phosphorylate the D3-hydroxyls of the inositol ring of phosphoinositides, and are responsible for coordinating a diverse range of cellular functions. A canonical pathway of activation of PI3Ks through the interaction of RA-domain with Ras proteins has been well established. In retinal photoreceptors, we have identified a non-canonical pathway of PI3Kγ activation through the interaction of its RA-domain with a putative Ras-like domain (RLD) in alpha subunit of cyclic nucleotide-gated channel (CNGA1) in retinal rod photoreceptors.Results: The interaction between PI3Kγ and CNGA1 does not appear to play a role in regulation of CNG channel activity, but PI3Kγ uses CNGA1 as an anchoring module to achieve close proximity to its substrate to generate D3-phosphoinositides.Conclusions: Our studies suggest a functional non-canonical PI3Kγ activation in retinal rod photoreceptor cells.

UR - https://www.scopus.com/pages/publications/84928682340

U2 - 10.1186/s12964-015-0087-9

DO - 10.1186/s12964-015-0087-9

M3 - Article

C2 - 25644171

AN - SCOPUS:84928682340

SN - 1478-811X

VL - 13

SP - 1

EP - 11

JO - Cell Communication and Signaling

JF - Cell Communication and Signaling

M1 - 7

ER -

Non-canonical regulation of phosphatidylinositol 3-kinase gamma isoform activity in retinal rod photoreceptor cells

Abstract

Access to Document

Other files and links

Fingerprint

Cite this