Non-invasive monitoring of cytokine-based regenerative treatment of cartilage by hyperspectral unmixing

Saabah B. Mahbub; Peter Succar; Martin E. Gosnell; Ayad G. Anwer; Benjamin Herbert; Ewa M. Goldys; Graham Vesey

doi:10.1117/12.2212336

Non-invasive monitoring of cytokine-based regenerative treatment of cartilage by hyperspectral unmixing

Saabah B. Mahbub, Peter Succar, Martin E. Gosnell, Ayad G. Anwer, Benjamin Herbert, Ewa M. Goldys, Graham Vesey

Research output: Chapter in Book/Report/Conference proceeding › Other chapter contribution

Abstract

Extracting biochemical information from tissue autofluorescence is a promising approach to non-invasively monitor disease treatments at a cellular level, without using any external biomarkers. Our recently developed unsupervised hyperspectral unmixing by Dependent Component Analysis (DECA) provides robust and detailed metabolic information with proper account of intrinsic cellular heterogeneity. Moreover this method is compatible with established methods of fluorescent biomarker labelling. Recently adipose-derived stem cell (ADSC) – based therapies have been introduced for treating different diseases in animals and humans. ADSC have been shown promise in regenerative treatments for osteoarthritis and other bone and joint disorders. One of the mechanism of their action is their anti-inflammatory effects within osteoarthritic joints which aid the regeneration of cartilage. These therapeutic effects are known to be driven by secretions of different cytokines from the ADSCs. We have been using the hyperspectral unmixing techniques to study in-vitro the effects of ADSC-derived cytokine-rich secretions with the cartilage chip in both human and bovine samples. The study of metabolic effects of different cytokine treatment on different cartilage layers makes it possible to compare the merits of those treatments for repairing cartilage.

Original language	English
Title of host publication	Progress in Biomedical Optics and Imaging
Subtitle of host publication	Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIV : proceedings
Editors	Tuan Vo-Dinh, Anita Mahadevan-Jansen, Warren S. Grundfest
Place of Publication	Bellingham, Washington
Publisher	SPIE
Pages	96980B-1-96980B-1
Number of pages	1
ISBN (Print)	9781628419320
DOIs	https://doi.org/10.1117/12.2212336
Publication status	Published - 2016
Event	Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIV (2016) - San Francisco, California, United States Duration: 14 Feb 2016 → 16 Feb 2016

Publication series

Name	Proceedings of SPIE
Publisher	SPIE
Volume	9698
ISSN (Print)	1605-7422

Conference

Conference	Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIV (2016)
City	San Francisco, California, United States
Period	14/02/16 → 16/02/16

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10.1117/12.2212336

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Mahbub, S. B., Succar, P., Gosnell, M. E., Anwer, A. G., Herbert, B., Goldys, E. M., & Vesey, G. (2016). Non-invasive monitoring of cytokine-based regenerative treatment of cartilage by hyperspectral unmixing. In T. Vo-Dinh, A. Mahadevan-Jansen, & W. S. Grundfest (Eds.), Progress in Biomedical Optics and Imaging: Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIV : proceedings (pp. 96980B-1-96980B-1). (Proceedings of SPIE; Vol. 9698). SPIE. https://doi.org/10.1117/12.2212336

Mahbub, Saabah B. ; Succar, Peter ; Gosnell, Martin E. et al. / Non-invasive monitoring of cytokine-based regenerative treatment of cartilage by hyperspectral unmixing. Progress in Biomedical Optics and Imaging: Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIV : proceedings. editor / Tuan Vo-Dinh ; Anita Mahadevan-Jansen ; Warren S. Grundfest. Bellingham, Washington : SPIE, 2016. pp. 96980B-1-96980B-1 (Proceedings of SPIE).

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title = "Non-invasive monitoring of cytokine-based regenerative treatment of cartilage by hyperspectral unmixing",

abstract = "Extracting biochemical information from tissue autofluorescence is a promising approach to non-invasively monitor disease treatments at a cellular level, without using any external biomarkers. Our recently developed unsupervised hyperspectral unmixing by Dependent Component Analysis (DECA) provides robust and detailed metabolic information with proper account of intrinsic cellular heterogeneity. Moreover this method is compatible with established methods of fluorescent biomarker labelling. Recently adipose-derived stem cell (ADSC) – based therapies have been introduced for treating different diseases in animals and humans. ADSC have been shown promise in regenerative treatments for osteoarthritis and other bone and joint disorders. One of the mechanism of their action is their anti-inflammatory effects within osteoarthritic joints which aid the regeneration of cartilage. These therapeutic effects are known to be driven by secretions of different cytokines from the ADSCs. We have been using the hyperspectral unmixing techniques to study in-vitro the effects of ADSC-derived cytokine-rich secretions with the cartilage chip in both human and bovine samples. The study of metabolic effects of different cytokine treatment on different cartilage layers makes it possible to compare the merits of those treatments for repairing cartilage.",

author = "Mahbub, {Saabah B.} and Peter Succar and Gosnell, {Martin E.} and Anwer, {Ayad G.} and Benjamin Herbert and Goldys, {Ewa M.} and Graham Vesey",

year = "2016",

doi = "10.1117/12.2212336",

language = "English",

isbn = "9781628419320",

series = "Proceedings of SPIE",

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Mahbub, SB, Succar, P, Gosnell, ME, Anwer, AG, Herbert, B, Goldys, EM & Vesey, G 2016, Non-invasive monitoring of cytokine-based regenerative treatment of cartilage by hyperspectral unmixing. in T Vo-Dinh, A Mahadevan-Jansen & WS Grundfest (eds), Progress in Biomedical Optics and Imaging: Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIV : proceedings. Proceedings of SPIE, vol. 9698, SPIE, Bellingham, Washington, pp. 96980B-1-96980B-1, Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIV (2016), San Francisco, California, United States, 14/02/16. https://doi.org/10.1117/12.2212336

Non-invasive monitoring of cytokine-based regenerative treatment of cartilage by hyperspectral unmixing. / Mahbub, Saabah B.; Succar, Peter; Gosnell, Martin E. et al.
Progress in Biomedical Optics and Imaging: Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIV : proceedings. ed. / Tuan Vo-Dinh; Anita Mahadevan-Jansen; Warren S. Grundfest. Bellingham, Washington: SPIE, 2016. p. 96980B-1-96980B-1 (Proceedings of SPIE; Vol. 9698).

Research output: Chapter in Book/Report/Conference proceeding › Other chapter contribution

TY - CHAP

T1 - Non-invasive monitoring of cytokine-based regenerative treatment of cartilage by hyperspectral unmixing

AU - Mahbub, Saabah B.

AU - Succar, Peter

AU - Gosnell, Martin E.

AU - Anwer, Ayad G.

AU - Herbert, Benjamin

AU - Goldys, Ewa M.

AU - Vesey, Graham

PY - 2016

Y1 - 2016

N2 - Extracting biochemical information from tissue autofluorescence is a promising approach to non-invasively monitor disease treatments at a cellular level, without using any external biomarkers. Our recently developed unsupervised hyperspectral unmixing by Dependent Component Analysis (DECA) provides robust and detailed metabolic information with proper account of intrinsic cellular heterogeneity. Moreover this method is compatible with established methods of fluorescent biomarker labelling. Recently adipose-derived stem cell (ADSC) – based therapies have been introduced for treating different diseases in animals and humans. ADSC have been shown promise in regenerative treatments for osteoarthritis and other bone and joint disorders. One of the mechanism of their action is their anti-inflammatory effects within osteoarthritic joints which aid the regeneration of cartilage. These therapeutic effects are known to be driven by secretions of different cytokines from the ADSCs. We have been using the hyperspectral unmixing techniques to study in-vitro the effects of ADSC-derived cytokine-rich secretions with the cartilage chip in both human and bovine samples. The study of metabolic effects of different cytokine treatment on different cartilage layers makes it possible to compare the merits of those treatments for repairing cartilage.

AB - Extracting biochemical information from tissue autofluorescence is a promising approach to non-invasively monitor disease treatments at a cellular level, without using any external biomarkers. Our recently developed unsupervised hyperspectral unmixing by Dependent Component Analysis (DECA) provides robust and detailed metabolic information with proper account of intrinsic cellular heterogeneity. Moreover this method is compatible with established methods of fluorescent biomarker labelling. Recently adipose-derived stem cell (ADSC) – based therapies have been introduced for treating different diseases in animals and humans. ADSC have been shown promise in regenerative treatments for osteoarthritis and other bone and joint disorders. One of the mechanism of their action is their anti-inflammatory effects within osteoarthritic joints which aid the regeneration of cartilage. These therapeutic effects are known to be driven by secretions of different cytokines from the ADSCs. We have been using the hyperspectral unmixing techniques to study in-vitro the effects of ADSC-derived cytokine-rich secretions with the cartilage chip in both human and bovine samples. The study of metabolic effects of different cytokine treatment on different cartilage layers makes it possible to compare the merits of those treatments for repairing cartilage.

U2 - 10.1117/12.2212336

DO - 10.1117/12.2212336

M3 - Other chapter contribution

SN - 9781628419320

T3 - Proceedings of SPIE

SP - 96980B-1-96980B-1

BT - Progress in Biomedical Optics and Imaging

A2 - Vo-Dinh, Tuan

A2 - Mahadevan-Jansen, Anita

A2 - Grundfest, Warren S.

PB - SPIE

CY - Bellingham, Washington

T2 - Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIV (2016)

Y2 - 14 February 2016 through 16 February 2016

ER -

Mahbub SB, Succar P, Gosnell ME, Anwer AG, Herbert B, Goldys EM et al. Non-invasive monitoring of cytokine-based regenerative treatment of cartilage by hyperspectral unmixing. In Vo-Dinh T, Mahadevan-Jansen A, Grundfest WS, editors, Progress in Biomedical Optics and Imaging: Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIV : proceedings. Bellingham, Washington: SPIE. 2016. p. 96980B-1-96980B-1. (Proceedings of SPIE). doi: 10.1117/12.2212336

Non-invasive monitoring of cytokine-based regenerative treatment of cartilage by hyperspectral unmixing

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