TY - JOUR
T1 - Nuclear proteome analysis of monkey embryonic stem cells during differentiation
AU - Nasrabadi, Davood
AU - Larijani, Mehran Rezaei
AU - Fathi, Ali
AU - Gourabi, Hamid
AU - Dizaj, Ahmad V.
AU - Baharvand, Hossein
AU - Salekdeh, Ghasem Hosseini
PY - 2010/3/1
Y1 - 2010/3/1
N2 - The nuclear proteome enables, manages, and regulates the genome by the collective actions and interactions of proteins found in the nucleus. We applied a proteomic approach to analyze a nuclear proteome during embryonic stem cell (ESC) proliferation, and 3 and 9 days after initiation of differentiation. The nuclei were isolated from cells and their proteins were separated using 2-DE. Out of about 560 protein spots reproducible detected on any give gel, 49 differentially expressed proteins were identified by Matrix Assisted Laser Desorption Ionization-Time of Flight (MALDI TOF/TOF) mass spectrometry. Of them, several nuclear located proteins involved in chromatin remodeling, transcription regulation, apoptosis, cell proliferation, and differentiation were identified including CTBP1, MM-1, RUVBL1, HCC-1, SGTA, SUMO2, and Galectin-1. Functional interaction analysis of differentially expressed proteins revealed that most of nuclear proteins had a direct interaction with c-Myc and p53.
AB - The nuclear proteome enables, manages, and regulates the genome by the collective actions and interactions of proteins found in the nucleus. We applied a proteomic approach to analyze a nuclear proteome during embryonic stem cell (ESC) proliferation, and 3 and 9 days after initiation of differentiation. The nuclei were isolated from cells and their proteins were separated using 2-DE. Out of about 560 protein spots reproducible detected on any give gel, 49 differentially expressed proteins were identified by Matrix Assisted Laser Desorption Ionization-Time of Flight (MALDI TOF/TOF) mass spectrometry. Of them, several nuclear located proteins involved in chromatin remodeling, transcription regulation, apoptosis, cell proliferation, and differentiation were identified including CTBP1, MM-1, RUVBL1, HCC-1, SGTA, SUMO2, and Galectin-1. Functional interaction analysis of differentially expressed proteins revealed that most of nuclear proteins had a direct interaction with c-Myc and p53.
KW - Differentiation
KW - Embryonic stem cells
KW - Monkey
KW - Nucleus
KW - Proteomics
UR - http://www.scopus.com/inward/record.url?scp=77949304086&partnerID=8YFLogxK
U2 - 10.1007/s12015-009-9109-6
DO - 10.1007/s12015-009-9109-6
M3 - Article
C2 - 20091144
AN - SCOPUS:77949304086
SN - 1550-8943
VL - 6
SP - 50
EP - 61
JO - Stem Cell Reviews and Reports
JF - Stem Cell Reviews and Reports
IS - 1
ER -