The effects of multiple mesoscale convective systems (MCSs) on the formation of Typhoon Ketsana (2003) are analyzed in this study. Numerical simulations using the Weather Research and Forecasting (WRF) model with assimilation of Quick Scatterometer (QuikSCAT) and Special Sensor Microwave Imager (SSM/I) oceanic winds and total precipitable water are performed. The WRF model simulates well the large-scale features, the convective episodes associated with the MCSs and their periods of development, and the formation time and location of Ketsana. With the successive occurrence of MCSs, midlevel average relative vorticity is strengthened through generation of mesoscale convective vortices (MCVs) mainly via the vertical stretching mechanism. Scale separation shows that the activity of the vortical hot tower (VHT)-type mesog-scale vortices correlated well with the development of the MCSs. These VHTs have large values of positive relative vorticity induced by intense low-level convergence, and thus play an important role in the low-level vortex enhancement with aggregation of VHTs as one of the possible mechanisms. Four sensitivity experiments are performed to analyze the possible different roles of the MCSs during the formation of Ketsana by modifying the vertical relative humidity profile in each MCS and consequently the strength of convection within. The results show that the development of an MCS depends substantially on that of the prior ones through remoistening of the midtroposphere, and thus leading to different scenarios of system intensification during the tropical cyclone (TC) formation. The earlier MCSs are responsible for the first stage vortex enhancement, and depending on the location can affect quite largely the simulated formation location. The extreme convection within the last MCS before formation largely determines the formation time.