O-GlcNAc regulated arginine methylation

a novel paradigm in survival signaling

Natasha Zachara, Kamau Fahie, Roger Henry, Devin Miller, Albert Lee

Research output: Contribution to journalMeeting abstract

Abstract

The modification of intracellular proteins by monosaccharides of O-linked β-N-acetylglucosamine (O-GlcNAc) has emerged as a regulator of cytoprotection. In response to cellular stress and injury, O-GlcNAc levels are elevated on numerous proteins. Notably, elevating O-GlcNAc levels before or after the induction of injury is protective in both in vivo and in vitro models. However, the molecular mechanisms by which O-GlcNAc protects injured cells/tissues remain elusive. In this study, we report that protein arginine methyltransferase 1 (PRMT1) is O-GlcNAc modified and associates with the O-GlcNAc transferase (OGT). Our data demonstrates that the association of OGT with PRMT1 inhibits methyltransferase activity. As a consequence, lowering OGT expression leads to an elevation of arginine assymetric dimethylation in vivo. Enhanced arginine assymetric dimethylation is associated with the development of cardiovascular disease, reduced lifespan, and sensitivity to apoptotic stimuli. Consistent with a model in which OGT inhibits PRMT1 leading to a stress-tolerant phenotype, the sensitivity of cells with reduced O-GlcNAcylation to oxidative stress can be ablated by PRMT1 knockdown or inhibition. Together, these data highlight one mechanism by which O-GlcNAc/OGT regulates cell survival and demonstrates that OGT may be as important as its catalytic activity in promoting cell survival during injury.
Original languageEnglish
Article number359.3
Number of pages1
JournalFASEB Journal
Volume28
Issue number1
Publication statusPublished - Apr 2014
Externally publishedYes
EventExperimental Biology Meeting - San Diego, United States
Duration: 26 Apr 201430 Apr 2014

Cite this

Zachara, N., Fahie, K., Henry, R., Miller, D., & Lee, A. (2014). O-GlcNAc regulated arginine methylation: a novel paradigm in survival signaling. FASEB Journal, 28(1), [359.3].