OlsG (Sinac_1600) is an ornithine lipid N-methyltransferase from the planctomycete Singulisphaera acidiphila

Wendy Itzel Escobedo-Hinojosa, Miguel Ángel Vences-Guzmán, Florence Schubotz, Mario Sandoval-Calderón, Roger E. Summons, Isabel María López-Lara, Otto Geiger, Christian Sohlenkamp*

*Corresponding author for this work

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Ornithine lipids (OLs) are phosphorus-free membrane lipids widespread in bacteria but absent from archaea and eukaryotes. In addition to the unmodified OLs, a variety of OL derivatives hydroxylated in different structural positions has been reported. Recently, methylated derivatives of OLs were described in several planctomycetes isolated from a peat bog in Northern Russia, although the gene/enzyme responsible for the N-methylation of OL remained obscure. Here we identify and characterize the OL N-methyltransferase OlsG (Sinac-1600) from the planctomycete Singulisphaera acidiphila. When OlsG is co-expressed with the OL synthase OlsF in Escherichia coli, methylated OL derivatives are formed. An in vitro characterization shows that OlsG is responsible for the 3-fold methylation of the terminal δ-nitrogen of OL. Methylation is dependent on the presence of the detergent Triton X-100 and the methyldonor S-adenosylmethionine.

Original languageEnglish
Pages (from-to)15102-15111
Number of pages10
JournalJournal of Biological Chemistry
Volume290
Issue number24
DOIs
Publication statusPublished - 12 Jun 2015
Externally publishedYes

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    Escobedo-Hinojosa, W. I., Vences-Guzmán, M. Á., Schubotz, F., Sandoval-Calderón, M., Summons, R. E., López-Lara, I. M., ... Sohlenkamp, C. (2015). OlsG (Sinac_1600) is an ornithine lipid N-methyltransferase from the planctomycete Singulisphaera acidiphila. Journal of Biological Chemistry, 290(24), 15102-15111. https://doi.org/10.1074/jbc.M115.639575