Oncogene-induced senescence does not require the p16 INK4a or p14ARF melanoma tumor suppressors

Sebastian Haferkamp, Lyndee L. Scurr, Therese M. Becker, Monika Frausto, Richard F. Kefford, Helen Rizos*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

57 Citations (Scopus)

Abstract

Oncogene-induced senescence is considered to act as a potent barrier to cell transformation, and has been seen in vivo during the early stages of tumor development. Human nevus cells frequently express oncogenic N-RAS or B-RAF, and are thought to be permanently growth arrested. Many studies have suggested that the p16 INK4a and, to a lesser extent, the p14ARF tumor suppressor proteins act as critical triggers of oncogene-induced senescence in nevi, and thus these proteins represent major inhibitors of progression to melanoma. There have also been reports, however, showing that p16 INK4a and/or p14ARF is not sufficient to execute the oncogene-induced senescence program. In this study, we examined the impact of melanoma-associated N-RAS Q61K on melanocyte senescence and utilized RNA-interference vectors to directly assess the individual contribution of human p14ARF and p16 INK4a genes to the N-RAS-induced senescence program. We formally show that cultured human melanocytes can initiate an effective oncogene-mediated senescence program in the absence of INK4a/ARF-encoded proteins. Our data are consistent with observations showing that senescent nevus cells do not always express p16 INK4a, and highlight the need to thoroughly explore INK4a/ARF-independent molecular pathways of senescence in human melanocytes.

Original languageEnglish
Pages (from-to)1983-1991
Number of pages9
JournalJournal of Investigative Dermatology
Volume129
Issue number8
DOIs
Publication statusPublished - Aug 2009
Externally publishedYes

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