Optogenetics, the intersection between physics and neuroscience: light stimulation of neurons in physiological conditions

Wei Deng, Ewa M. Goldys, Melissa M J Farnham, Paul M. Pilowsky

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Neuronal stimulation by light is a novel approach in the emerging field of optogenetics, where genetic engineering is used to introduce light-activated channels. However, light is also capable of stimulating neurons even in the absence of genetic modifications through a range of physical and biological mechanisms. As a result, rigorous design of optogenetic experiments needs to take note of alternative and parallel effects of light illumination of neuronal tissues. Thus all matters relating to light penetration are critical to the development of studies using light-activated proteins. This paper discusses ways to quantify light, light penetration in tissue, as well as light stimulation of neurons in physiological conditions. We also describe the direct effect of light on neurons investigated at different sites.

LanguageEnglish
PagesR1292-R1302
Number of pages11
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume307
Issue number11
DOIs
Publication statusPublished - 1 Dec 2014

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Optogenetics
Physics
Neurosciences
Neurons
Light
Genetic Engineering
Lighting

Cite this

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Optogenetics, the intersection between physics and neuroscience : light stimulation of neurons in physiological conditions. / Deng, Wei; Goldys, Ewa M.; Farnham, Melissa M J; Pilowsky, Paul M.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 307, No. 11, 01.12.2014, p. R1292-R1302.

Research output: Contribution to journalArticleResearchpeer-review

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