Organellar proteomics of embryonic stem cells

Faezeh Shekari, Hossein Baharvand, Ghasem Hosseini Salekdeh

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

Abstract

Embryonic stem cells (ESCs) are undifferentiated cells with two common remarkable features known as self-renewal and differentiation. Proteomics plays an increasingly important role in understanding molecular mechanisms underlying self-renewal and pluripotency of ESCs and their applications in cell therapy and developmental biology studies. As the function of a protein is strongly associated with its localization in cell, a complete and accurate picture of the proteome of ESCs cannot be achieved without knowing the subcellular locations of proteins. Subcellular fractionation allows enrichment of low abundant proteins and signaling complexes and reduces the complexity of the sample. It also provided insight into tracking proteins that shuttle between different compartments. Despite the substantial interest and efforts in ESC subcellular proteomics area, progress has been relatively limited. In this review, we present an overview on current status of ESCs organelle proteomics research and discuss challenges in subcellular proteomics.

LanguageEnglish
Title of host publicationProteomics in biomedicine and pharmacology
EditorsRossen Donev
Place of PublicationUnites States
PublisherElsevier Academic Press
Chapter7
Pages215-230
Number of pages16
ISBN (Print)9780128004531
DOIs
Publication statusPublished - 1 Jan 2014
Externally publishedYes

Publication series

NameAdvances in Protein Chemistry and Structural Biology
Volume95
ISSN (Print)1876-1623

Fingerprint

Embryonic Stem Cells
Stem cells
Proteomics
Proteins
Developmental Biology
Proteome
Fractionation
Cell- and Tissue-Based Therapy
Organelles
Cell Biology
Research

Keywords

  • Embryonic stem cells
  • Fractionation
  • Organelle proteomics
  • Proteomics

Cite this

Shekari, F., Baharvand, H., & Salekdeh, G. H. (2014). Organellar proteomics of embryonic stem cells. In R. Donev (Ed.), Proteomics in biomedicine and pharmacology (pp. 215-230). (Advances in Protein Chemistry and Structural Biology; Vol. 95). Unites States: Elsevier Academic Press. https://doi.org/10.1016/B978-0-12-800453-1.00007-5
Shekari, Faezeh ; Baharvand, Hossein ; Salekdeh, Ghasem Hosseini. / Organellar proteomics of embryonic stem cells. Proteomics in biomedicine and pharmacology. editor / Rossen Donev. Unites States : Elsevier Academic Press, 2014. pp. 215-230 (Advances in Protein Chemistry and Structural Biology).
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Shekari, F, Baharvand, H & Salekdeh, GH 2014, Organellar proteomics of embryonic stem cells. in R Donev (ed.), Proteomics in biomedicine and pharmacology. Advances in Protein Chemistry and Structural Biology, vol. 95, Elsevier Academic Press, Unites States, pp. 215-230. https://doi.org/10.1016/B978-0-12-800453-1.00007-5

Organellar proteomics of embryonic stem cells. / Shekari, Faezeh; Baharvand, Hossein; Salekdeh, Ghasem Hosseini.

Proteomics in biomedicine and pharmacology. ed. / Rossen Donev. Unites States : Elsevier Academic Press, 2014. p. 215-230 (Advances in Protein Chemistry and Structural Biology; Vol. 95).

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

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Shekari F, Baharvand H, Salekdeh GH. Organellar proteomics of embryonic stem cells. In Donev R, editor, Proteomics in biomedicine and pharmacology. Unites States: Elsevier Academic Press. 2014. p. 215-230. (Advances in Protein Chemistry and Structural Biology). https://doi.org/10.1016/B978-0-12-800453-1.00007-5