Organellar proteomics of embryonic stem cells

Faezeh Shekari, Hossein Baharvand*, Ghasem Hosseini Salekdeh

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Citations (Scopus)

Abstract

Embryonic stem cells (ESCs) are undifferentiated cells with two common remarkable features known as self-renewal and differentiation. Proteomics plays an increasingly important role in understanding molecular mechanisms underlying self-renewal and pluripotency of ESCs and their applications in cell therapy and developmental biology studies. As the function of a protein is strongly associated with its localization in cell, a complete and accurate picture of the proteome of ESCs cannot be achieved without knowing the subcellular locations of proteins. Subcellular fractionation allows enrichment of low abundant proteins and signaling complexes and reduces the complexity of the sample. It also provided insight into tracking proteins that shuttle between different compartments. Despite the substantial interest and efforts in ESC subcellular proteomics area, progress has been relatively limited. In this review, we present an overview on current status of ESCs organelle proteomics research and discuss challenges in subcellular proteomics.

Original languageEnglish
Title of host publicationProteomics in biomedicine and pharmacology
EditorsRossen Donev
Place of PublicationUnites States
PublisherElsevier Academic Press
Chapter7
Pages215-230
Number of pages16
ISBN (Print)9780128004531
DOIs
Publication statusPublished - 1 Jan 2014
Externally publishedYes

Publication series

NameAdvances in Protein Chemistry and Structural Biology
Volume95
ISSN (Print)1876-1623

Keywords

  • Embryonic stem cells
  • Fractionation
  • Organelle proteomics
  • Proteomics

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  • Cite this

    Shekari, F., Baharvand, H., & Salekdeh, G. H. (2014). Organellar proteomics of embryonic stem cells. In R. Donev (Ed.), Proteomics in biomedicine and pharmacology (pp. 215-230). (Advances in Protein Chemistry and Structural Biology; Vol. 95). Unites States: Elsevier Academic Press. https://doi.org/10.1016/B978-0-12-800453-1.00007-5