|Title of host publication||Encyclopedia of Biophysics|
|Editors||Gordon Roberts, Anthony Watts, European Biophysical Societies|
|Place of Publication||Berlin|
|Publisher||Springer, Springer Nature|
|Number of pages||2|
|Publication status||Published - 28 Jul 2018|
Oriented circular dichroism (OCD) spectroscopy, as the name implies, is the circular dichroism spectroscopy of oriented samples. It can be a very useful technique; however, it is prone to artifacts resulting from linear dichroism contributions to the observed spectrum (Nordén et al. 2010). Thus care must be taken in collecting and analyzing the data. A sample for OCD should be symmetric about a unique orientation axis. Data must then be collected with the light propagating along that unique axis and spectra measured at different rotations about the unique axis compared. If the rotated spectra are the same, then these data can be believed. If the sample is perfectly oriented (as it would be in a perfectly oriented crystal but probably in no other method), the observed spectrum is then equivalent to three times (no rotational averaging) the CD spectrum of all the transitions in an isotropic sample except those polarized along the unique axis (which are invisible).