Otx2 expression and implications for olfactory imprinting in the anemonefish, Amphiprion percula

Heather D. Veilleux; Lynne Van Herwerden; Nicholas J. Cole; Emily K. Don; Christian De Santis; Danielle L. Dixson; Amelia S. Wenger; Philip L. Munday

doi:10.1242/bio.20135496

Otx2 expression and implications for olfactory imprinting in the anemonefish, Amphiprion percula

Heather D. Veilleux^*, Lynne Van Herwerden, Nicholas J. Cole, Emily K. Don, Christian De Santis, Danielle L. Dixson, Amelia S. Wenger, Philip L. Munday

^*Corresponding author for this work

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Abstract

The otx2 gene encodes a transcription factor (OTX2) essential in the formation of the brain and sensory systems. Specifically, OTX2-positive cells are associated with axons in the olfactory system of mice and otx2 is upregulated in odour-exposed zebrafish, indicating a possible role in olfactory imprinting. In this study, otx2 was used as a candidate gene to investigate the molecular mechanisms of olfactory imprinting to settlement cues in the coral reef anemonefish, Amphiprion percula. The A. percula otx2 (Apotx2) gene was elucidated, validated, and its expression tested in settlement-stage A. percula by exposing them to behaviourally relevant olfactory settlement cues in the first 24 hours post-hatching, or daily throughout the larval phase. In-situ hybridisation revealed expression of Ap-otx2 throughout the olfactory epithelium with increased transcript staining in odour-exposed settlement-stage larval fish compared to no-odour controls, in all scenarios. This suggests that Ap-otx2 may be involved in olfactory imprinting to behaviourally relevant settlement odours in A. percula.

Original language	English
Pages (from-to)	907-915
Number of pages	9
Journal	Biology Open
Volume	2
Issue number	9
DOIs	https://doi.org/10.1242/bio.20135496
Publication status	Published - 15 Sept 2013

Bibliographical note

Copyright the Author(s) 2013. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

Olfaction
Coral reef fish
Habitat selection
Memory
Candidate gene
In-situ hybridisation

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10.1242/bio.20135496

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title = "Otx2 expression and implications for olfactory imprinting in the anemonefish, Amphiprion percula",

abstract = "The otx2 gene encodes a transcription factor (OTX2) essential in the formation of the brain and sensory systems. Specifically, OTX2-positive cells are associated with axons in the olfactory system of mice and otx2 is upregulated in odour-exposed zebrafish, indicating a possible role in olfactory imprinting. In this study, otx2 was used as a candidate gene to investigate the molecular mechanisms of olfactory imprinting to settlement cues in the coral reef anemonefish, Amphiprion percula. The A. percula otx2 (Apotx2) gene was elucidated, validated, and its expression tested in settlement-stage A. percula by exposing them to behaviourally relevant olfactory settlement cues in the first 24 hours post-hatching, or daily throughout the larval phase. In-situ hybridisation revealed expression of Ap-otx2 throughout the olfactory epithelium with increased transcript staining in odour-exposed settlement-stage larval fish compared to no-odour controls, in all scenarios. This suggests that Ap-otx2 may be involved in olfactory imprinting to behaviourally relevant settlement odours in A. percula.",

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AU - De Santis, Christian

AU - Dixson, Danielle L.

AU - Wenger, Amelia S.

AU - Munday, Philip L.

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Otx2 expression and implications for olfactory imprinting in the anemonefish, Amphiprion percula

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