Otx2 expression and implications for olfactory imprinting in the anemonefish, Amphiprion percula

Heather D. Veilleux*, Lynne Van Herwerden, Nicholas J. Cole, Emily K. Don, Christian De Santis, Danielle L. Dixson, Amelia S. Wenger, Philip L. Munday

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Citation (Scopus)
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Abstract

The otx2 gene encodes a transcription factor (OTX2) essential in the formation of the brain and sensory systems. Specifically, OTX2-positive cells are associated with axons in the olfactory system of mice and otx2 is upregulated in odour-exposed zebrafish, indicating a possible role in olfactory imprinting. In this study, otx2 was used as a candidate gene to investigate the molecular mechanisms of olfactory imprinting to settlement cues in the coral reef anemonefish, Amphiprion percula. The A. percula otx2 (Apotx2) gene was elucidated, validated, and its expression tested in settlement-stage A. percula by exposing them to behaviourally relevant olfactory settlement cues in the first 24 hours post-hatching, or daily throughout the larval phase. In-situ hybridisation revealed expression of Ap-otx2 throughout the olfactory epithelium with increased transcript staining in odour-exposed settlement-stage larval fish compared to no-odour controls, in all scenarios. This suggests that Ap-otx2 may be involved in olfactory imprinting to behaviourally relevant settlement odours in A. percula.

Original languageEnglish
Pages (from-to)907-915
Number of pages9
JournalBiology Open
Volume2
Issue number9
DOIs
Publication statusPublished - 15 Sep 2013

Bibliographical note

Copyright the Author(s) 2013. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • Olfaction
  • Coral reef fish
  • Habitat selection
  • Memory
  • Candidate gene
  • In-situ hybridisation

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