Overview of gene expression using filamentous fungi

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Filamentous fungi are lower eukaryotes increasingly used for expression of foreign proteins ranging from industrial enzymes originating from other fungi and bacteria to proteins of mammalian origin, such as antibodies and growth factors. Their strengths include an excellent capacity for protein secretion and their eukaryotic protein processing machinery. Proteins secreted from filamentous fungi are modified in the secretory pathway, with folding, proteolytic processing, and addition of glycans being the main modifications. Unlike from many other expression systems, however, plasmids and host strains for expression of gene products in filamentous fungi are not readily available commercially, and the expression system must thus be stitched together in the laboratory. In this overview, the key elements of fungal expression systems are discussed from a practical point of view and with a view towards the future. The principles and considerations presented here can be applied to a range of filamentous fungi.

LanguageEnglish
Article numbere55
Pages1-10
Number of pages10
JournalCurrent Protocols in Protein Science
Volume92
Issue number1
DOIs
Publication statusPublished - Apr 2018

Fingerprint

Fungi
Gene expression
Gene Expression
Proteins
Secretory Pathway
Processing
Eukaryota
Machinery
Polysaccharides
Intercellular Signaling Peptides and Proteins
Bacteria
Plasmids
Genes
Antibodies
Enzymes

Keywords

  • filamentous fungi
  • gene expression
  • gene promoters
  • protein secretion
  • post-translational protein processing
  • protease deficient hosts

Cite this

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Overview of gene expression using filamentous fungi. / Nevalainen, Helena; Peterson, Robyn; Curach, Natalie.

In: Current Protocols in Protein Science, Vol. 92, No. 1, e55, 04.2018, p. 1-10.

Research output: Contribution to journalArticleResearchpeer-review

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