A detailed palynological sequence of the late Middle Palaeolithic (MP) open-air site Nahal Mahanayeem Outlet (NMO), northern Jordan Rift Valley, Israel, dated to ca. 65-51 ka, constitutes a rare case of a south Levantine archaeo-palynological study for this period. The sequence is characterized by relatively high Arboreal Pollen (AP) ratios, comparable to humid fluctuations in the near-by Hula cores. The pollen spectra represent a Mediterranean maquis with Quercus calliprinos (Palestine oak), Quercus ithaburensis (Mt. Tabor oak), Pistacia sp. (pistachio), Pinus halepensis (Aleppo pine), and Olea europaea (wild olive) as the main tree taxa. Among the Non Arboreal Pollen (NAP) the dominant families are Poaceae (true grasses family), Chenopodiaceae (goosefoot family), Apiaceae (parsley family) and Liliaceae (lily family), and the genera Artemisia (sagebrush) and Centaurea (centaury). Two humid fluctuations with higher AP ratios, separated by a somewhat drier one, seem to emerge. The earlier humid fluctuation represents a cooler climate as indicated by the occurrence of Cedrus libani (Lebanon cedar), Abies (fir), and Alnus (alder) pollen. Altogether, the palynological data suggest wet habitats near the site in a rather humid period, when a Mediterranean maquis was widespread on the mountains surrounding the valley, with some minor fluctuations in humidity and temperature. Results of the current study fit those of a previous study of the Hula Basin for roughly the same time period. They further provide valuable details and a unique palaeoenvironmental reconstruction specifically related to the occupation period of NMO. Given the postulated short-term nature of its human occupation, the palynological sequence may represent a longer period of accumulation, extending before, during and after human presence at the site, with short human habitation spell/s incorporated within the sequence. The unique preservation of rich and diverse micro-botanical (pollen) and macro-botanical (wood, bark, seeds and fruits) assemblages at the site further contributes to the comprehensive reconstruction of a variety of biotopes exploited by the inhabitants of the site, both in the vicinity and further afield.