Pathway Tools version 13.0: Integrated software for pathway/genome informatics and systems biology

Peter D. Karp*, Suzanne M. Paley, Markus Krummenacker, Mario Latendresse, Joseph M. Dale, Thomas J. Lee, Pallavi Kaipa, Fred Gilham, Aaron Spaulding, Liviu Popescu, Tomer Altman, Ian Paulsen, Ingrid M. Keseler, Ron Caspi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

342 Citations (Scopus)


Pathway Tools is a production-quality software environment for creating a type of model-organism database called a Pathway/Genome Database (PGDB). A PGDB such as EcoCyc integrates the evolving understanding of the genes, proteins, metabolic network and regulatory network of an organism. This article provides an overview of Pathway Tools capabilities. The software performs multiple computational inferences including prediction of metabolic pathways, prediction of metabolic pathway hole fillers and prediction of operons. It enables interactive editing of PGDBs by DB curators. It supports web publishing of PGDBs, and provides a large number of query and visualization tools. The software also supports comparative analyses of PGDBs, and provides several systems biology analyses of PGDBs including reachability analysis of metabolic networks, and interactive tracing of metabolites through a metabolic network. More than 800 PGDBs have been created using Pathway Tools by scientists around the world, many of which are curated DBs for important model organisms. Those PGDBs can be exchanged using a peerto-peer DB sharing system called the PGDB Registry.

Original languageEnglish
Article numberbbp043
Pages (from-to)40-79
Number of pages40
JournalBriefings in Bioinformatics
Issue number1
Publication statusPublished - 2 Dec 2009

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